冶金模式微生物Acidithiobacillus ferrooxidans表面质子吸附特性的研究

为了探究生物冶金过程及矿山酸性废水中的关键微生物Acidithiobacillus ferrooxidans的表面质子吸附机理，该研究通过酸碱滴定、ProtoFit模拟、电泳迁移率测定及傅里叶变换衰减全反射红外光谱法（ATR-FTIR）系统地揭示不同能源（S0、Fe2+、FeS₂）和离子强度（0.001~0.1 mol/L NaNO₃）对A.ferrooxidans表面性质的影响及参与质子化/去质子化反应的关键官能团。结果表明:三位点的Donnan壳静电模型能较好地适用于分析A.ferrooxidans的表面酸碱性质；能源和离子强度均影响A.ferrooxidans的表面酸碱性质；在广泛的pH值范围内（2~10），不同能源培养的A.ferrooxidans在不同离子强度下均呈负电性；羧基、磷酸基和酰胺基是质子化/去质子化过程的关键官能团。该研究表明了A.ferrooxidans在吸附重金属方面的重要应用潜力，并对阐明A.ferrooxidans的表面质子吸附机理具有重要意义。 

Study on Surface Proton Adsorption Characteristics of Bioleaching Model Microorganism Acidithiobacillus ferrooxidans

A. ferrooxidans is an important bioleaching microorganism and the main driving microorganism for the formation of acid mine drainage. To investigate the surface proton adsorption mechanism of A. ferrooxidans, acid-base titration, ProtoFit simulation, electrophoretic mobility determination and Fourier transform attenuated total reflection infrared spectroscopy (ATR-FTIR) were applied to study the effects of different energy sources (S0, Fe2+, FeS₂) and ionic strength (0.001 mol/L, 0.01 mol/L and 0.1 mol/L NaNO₃) on surface protonation/deprotonation of A. ferrooxidans and the key functional groups involved in the reaction. Results showed that three-site Donnan model could describe the surface complexation of A. ferrooxidans pretty well; The surface properties of A. ferrooxidans were sensitive to both culture energy and ionic strength; A. ferrooxidans was negatively charged in the broad pH range (2-9) studied; The carboxyl group, phosphate group and amide group are the key functional groups in the protonation/deprotonation process. This study indicates important application potential of A. ferrooxidans in the adsorption of heavy metals and has important guiding significance for the elucidation of the surface proton adsorption mechanism of A. ferrooxidans.