| Effects of Cerium on Accumulation of Anthocyanins and Expression of Anthocyanin Biosynthetic Genes in Potato Cell Tissue Cultures |
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| 作者姓名： | 卢其能 杨清 邹华文 |
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| 作者单位： | Department of Biochemistry and Molecular Biology College of Life Sciences，Nanjing Agricultural University Nanjing 210095 China，Department of Agronomy Yichun University Yichun 336000 China，Department of Biochemistry and Molecular Biology College of Life Sciences，Nanjing Agricultural University Nanjing 210095 China，Department of Biochemistry and Molecular Biology College of Life Sciences，Nanjing Agricultural University Nanjing 210095 China |
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| 基金项目： | 江苏省国际科技合作计划 |
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| 摘 要： | The effects of Ce (Ⅳ) on callus growth, anthocyanin content, and expression of anthocyanin biosynthetic genes in callus suspension cultures of Solanum tuberosum cv. Chieftain were studied by the measurement of fresh weight, spectrophotometric assays, and semiquantitative RT-PCR. The results indicate that 0.1 mmol·L-1 Ce (Ⅳ) can promote callus growth, increase the accumulation of anthocyanins, and enhance the expression of five anthocyanin biosynthetic genes (CHS, F3H, F3′5′H, DFR, and 3GT) most efficiently. At high concentrations of 1 mmol·L-1, Ce (Ⅳ) partially inhibits callus growth and at 2 mmol·L-1 eventually lends to cell death. The results show that Ce(Ⅳ) can induce the expression of anthocyanin biosynthetic genes to produce and accumulate anthocyanins and increase the yield of anthocyanins.
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| 关 键 词： | 花青素 铈 愈合组织 基因表达 稀土 |
| 文章编号： | 1002-0721（2006）04-0479-06 |
| 收稿时间： | 2006-01-05 |
| 修稿时间： | 2006-03-20 |
Effects of Cerium on Accumulation of Anthocyanins and Expression of Anthocyanin Biosynthetic Genes in Potato Cell Tissue Cultures |
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| Qineng , Qing Yang,Huawen Zou.Effects of Cerium on Accumulation of Anthocyanins and Expression of Anthocyanin Biosynthetic Genes in Potato Cell Tissue Cultures[J].Journal of Rare Earths,2006,24(4):479-484. |
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| Authors: | Qineng Qing Yang Huawen Zou |
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| Affiliation: | aDepartment of Biochemistry and Molecular Biology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China;bDepartment of Agronomy, Yichun University, Yichun 336000, China |
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| Abstract: | The effects of Ce (IV) on callus growth, anthocyanin content, and expression of anthocyanin biosynthetic genes in callus suspension cultures of Solanum tuberosum cv. Chieftain were studied by the measurement of fresh weight, spectrophotometric assays, and semiquantitative RT-PCR. The results indicate that 0.1 mmol·L−1 Ce (IV) can promote callus growth, increase the accumulation of anthocyanins, and enhance the expression of five anthocyanin biosynthetic genes (CHS, F3H, F3′5′H, DFR, and 3GT) most efficiently. At high concentrations of 1 mmol·L−1, Ce (IV) partially inhibits callus growth and at 2 mmol·L−1 eventually lends to cell death. The results show that Ce (IV) can induce the expression of anthocyanin biosynthetic genes to produce and accumulate anthocyanins and increase the yield of anthocyanins. |
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| Keywords: | anthocyanins cerium callus suspensive culture expression of genes Solanum tuberosum cv Chieftain rare earths |
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