| Comparison of Biochemical Effects Induced by Changle between Male and Female Rats Using NMR and ICP-MS Techniques |
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| 作者姓名： | 吴惠丰 李晓晶 冯江华 李伟生 李中峰 廖沛球 吴亦洁 裴奉奎 |
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| 作者单位： | Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China，Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China，Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China，Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China，Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China，Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China，Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China，Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China |
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| 摘 要： | NMRspectroscopyofbiofluidhasbroughtnew chemistryintolifescienceandclinicalmedicine,and NMRspectroscopy basedapproachhasbeensuccess fullyappliedtothestudiesofbiochemicaleffectsand inborndiseaseswiththeadventofhighfieldNMR spectrometers1].Inrecentyears,highresolutionNMRhasbeenusedtotherapidmulticomponentanal ysisoflowmolecularweightcompoundsinbiofluid,includingurine,bile,cerebrospinalfluid,saliva,milkandsynovialfluidforclinicaldiagnosisofinborn diseasesandinvestigationofbiochemicaleffectsof…
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| 关 键 词： | NMR ICP-MS 稀土 生物化学 生理 大鼠 新陈代谢 |
| 文章编号： | 1002-0721（2006）01-0108-05 |
| 收稿时间： | 2004-11-23 |
| 修稿时间： | 2005-04-12 |
Comparison of Biochemical Effects Induced by Changle between Male and Female Rats Using NMR and ICP-MS Techniques |
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| Huifeng Wu, Xiaojing Li, Jianghua Feng, Weisheng Li, Zhongfeng Li, Peiqiu Liao, Yijie ,Fengkui Pei,.Comparison of Biochemical Effects Induced by Changle between Male and Female Rats Using NMR and ICP-MS Techniques[J].Journal of Rare Earths,2006,24(1):108-114. |
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| Authors: | Huifeng Wu Xiaojing Li Jianghua Feng Weisheng Li Zhongfeng Li Peiqiu Liao Yijie Fengkui Pei |
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| Affiliation: | Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China |
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| Abstract: | Metabolic profiles caused by rare earth complex were investigated using NMR and ICP-MS techniques. Male and female Wistar rats were treated orally with Changle (A kind of rare earth complex applied in agriculture to raise the production of crops) at dose of 2, 5 and 20 mg*kg-1 body weight/day respectively for 90 d. Urine and serum samples are collected on 90 d. The relative concentrations of important endogenous metabolites in urine and serum are determined from 1H NMR spectra and the contents of the four rare earth elements (La, Ce, Pr and Nd) constituting Changle in the serum samples are measured by ICP-MS technique. Changle-induced renal and liver damage in rats is found based on the increase in the amounts of the amino acids, trimethylamine N-oxide, N, N-dimethyglycine, dimethylamine, succinate, α-ketoglutarate and ethanol as well as rare earth concentrations. The similarities and differentiations are found in the alteration patterns of metabolites and rare earth concentrations in serum. |
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| Keywords: | biofluid Changle NMR ICP-MS metabolism rare earths |
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