Genome-wide genetic diversity may help identify fine-scale genetic structure among lake whitefish spawning groups in Lake Erie |
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| Affiliation: | 1. Department of Forestry and Natural Resources, Purdue University, 715 W. State Street, West Lafayette, IN 47907-2061, USA;2. U.S. Geological Survey, Lake Erie Biological Station 380 Huron Street, Huron, OH 44839, USA;3. Lake Erie Management Unit, Ontario Ministry of Northern Development, Mines, Natural Resources and Forestry. RR # 2, 320 Milo Road, Wheatley ON, N0P 2P0, Canada;4. Lake Erie Fisheries Research Unit, New York State Department of Environmental Conservation, 178 Point Drive North, Dunkirk, NY 14048-1031, USA;1. Department of Biological Sciences, East Tennessee State University, Johnson City, TN, United States;2. Department of Ecology and Evolutionary Biology, Yale University, New Haven, CT, United States;1. National Oceanic and Atmospheric Administration, Great Lakes Environmental Research Laboratory, Lake Michigan Field Station, 1431 Beach St., Muskegon MI, 49441, USA;2. National Oceanic and Atmospheric Administration, Great Lakes Environmental Research Laboratory, 4840 S. State Rd., Ann Arbor, MI 48108, USA;1. Department of Landscape Architecture, University of Pennsylvania. 102 Meyerson Hall, 210 South 34th Street, Philadelphia, PA 19104, USA;2. Department of Landscape Architecture, University of Virginia, Charlottesville, VA, USA;1. U.S. Geological Survey (USGS), Great Lakes Science Center, 17 Lake St, Oswego, NY, USA;2. USGS, Great Lakes Science Center, 1451 Green Rd., Ann Arbor, MI, USA;3. USGS, Great Lakes Science Center, 3075 Gracie Road, Cortland, NY, USA;4. New York State Department of Environmental Conservation (NYSDEC), Cape Vincent Fisheries Station, 541 East Broadway St., Cape Vincent, NY, USA;5. U.S. Fish and Wildlife Service (USFWS), Northeast Fishery Center, Lamar, PA, USA;6. Great Lakes Fishery Commission, Ann Arbor, MI, USA;7. Ontario Ministry of Northern Development, Mines, Natural Resources and Forestry (NDMNRF), White Lake Fish Culture Station, 25900 Highway 7, Sharbot Lake, ON, Canada;8. University of Windsor, Great Lakes Institute for Environmental Research, Windsor, ON, Canada;9. USFWS, Jordan River National Fish Hatchery, 6623 Turner Road, Elmira, MI, USA;10. USFWS, Green Bay Fish and Wildlife Conservation Office, 2661 Scott Tower Drive, New Franken, WI, USA;11. NDMNRF, Fish Culture Section, Peterborough, ON, Canada;1. U.S. Fish and Wildlife Service, Lower Great Lakes Fish and Wildlife Conservation Office, 1101 Casey Road, Basom NY 14013, USA;2. New York Sea Grant, SUNY College at Oswego, Oswego, NY 13126-3599, USA;3. Rubenstein School of Environment and Natural Resources, University of Vermont, 81 Carrigan Dr., Burlington, VT 05405, USA;4. US Geological Survey, Lake Ontario Biological Station, 17 Lake St, Oswego, NY 13126, USA |
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| Abstract: | In Lake Erie, lake whitefish Coregonus clupeaformis supported lucrative fisheries before populations were decimated by overfishing and water quality degradation. In recent years, there has been a renewed interest in lake whitefish and management of the fishery they support. Lake whitefish spawn on several reefs throughout Lake Erie, but the relative recruitment dynamics and contributions of spawning groups to the fishery are not well understood. Modern high-throughput sequencing approaches offer new opportunities to census population diversity and to identify subtle differences among closely related populations. We used high-throughput sequencing data to evaluate the genetic structure and diversity of lake whitefish collected opportunistically across broad spatial scales in Lake Erie. Using RAD-capture (Rapture), we sequenced and genotyped individuals (N = 88) from the west, central, and east basin of Lake Erie at 120,268 single nucleotide polymorphisms (SNPs). Lake whitefish from Niagara and Crib Reefs (west basin) diverged from the three collections. Interestingly, these were the only lake whitefish collected during the act of spawning (late November), and all other fish were collected pre-spawn (August-early November). These results suggest that some lake whitefish spawning reefs may be reproductively isolated, though definition of these groups into stocks will require more intentional sampling during the act of spawning. |
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| Keywords: | Mixed-stock assignment Great Lakes Genetics Local adaptation Coregonidae RAD sequencing |
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