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耐辐射球菌对Cu2+的去除效率
引用本文:韩万春,李铭锋,田 兵,华跃进,卢振兰.耐辐射球菌对Cu2+的去除效率[J].水资源保护,2014(1):64-67.
作者姓名:韩万春  李铭锋  田 兵  华跃进  卢振兰
作者单位:[1]吉林农业大学资源与环境学院,吉林长春130118 [2]浙江大学原子核农业科学研究所,浙江杭州310029 [3]山东省东营市环境保护局,山东东营257000
基金项目:国家自然科学基金(30830006,31170079)
摘    要:耐辐射球菌是一种非致病菌,拥有极强的抗辐射、抗氧化特性,具有成为工程菌处理Cu2+的潜力。采用酶标仪测定Cu2+对耐辐射球菌的生长速度的影响,用电感耦合等离子光谱法测定菌液离心后得到的上清液Cu2+浓度。结果表明,当Cu2+初始质量浓度为1.36mg/L时,耐辐射球菌对其清除效率为57.3%;当-Cu2+初始质量浓度为6.28mg/L时,对其清除效率为35.4%。此外,用激光共聚焦显微镜观察受到Cu2+胁迫的耐辐射球菌,发现球菌具有明显的聚集效应。

关 键 词:耐辐射球菌Cu2+  电感耦合等离子光谱法  去除效率

Removal efficiency of Cu2+ by Deinococcus radiodurans
HAN Wanchun,LI Mingfeng,TIAN Bing,HUA Yuejin,LU Zhenlan.Removal efficiency of Cu2+ by Deinococcus radiodurans[J].Water Resources Protection,2014(1):64-67.
Authors:HAN Wanchun  LI Mingfeng  TIAN Bing  HUA Yuejin  LU Zhenlan
Institution:1. College of Resources and Environment, Jilin Agricultural University, Changchun 130118, China ; 2. Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou 310029, China; 3. Dongying Environmental Protection Agency of Shandong Province, Dongying 257000, China)
Abstract:As a non-pathogenic bacteria, Deinococcus radiodurans is extremely resistant to ionizing radiation and oxidative stress and shows great potential in remediation of Cu2+ pollution. In this study, we used the ELIASA plate reader and the ICP-OES, respectively, to monitor the growth rate of Deinococcus radiodurans and the Cu2+ concentration in the supernatant after the bacterial solution was centrifuged. The results show that the removal efficiency of Cu2+ by Deinococcus radiodurans reached 57.3% when the initial concentration of Cu2+ was 1.36mg/L, and the removal efficiency dropped to 35.4% when the initial concentration of Cu2+ increased to 6.28 mg/L. Meanwhile, using confocal laser scanning microscopy (CLSM), we observed that Deinococcus radiodurans assembled at a high concentration of Cu2+.
Keywords:Deinococcus radiodurans  Cu2+  ICP-OES  removal efficiency
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