| 耐辐射球菌对Cu2＋的去除效率 |
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| 引用本文： | 韩万春,李铭锋,田 兵,华跃进,卢振兰.耐辐射球菌对Cu2＋的去除效率[J].水资源保护,2014(1):64-67. |
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| 作者姓名： | 韩万春 李铭锋 田 兵 华跃进 卢振兰 |
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| 作者单位： | [1]吉林农业大学资源与环境学院,吉林长春130118 [2]浙江大学原子核农业科学研究所,浙江杭州310029 [3]山东省东营市环境保护局,山东东营257000 |
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| 基金项目： | 国家自然科学基金（30830006,31170079） |
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| 摘 要： | 耐辐射球菌是一种非致病菌，拥有极强的抗辐射、抗氧化特性，具有成为工程菌处理Cu2＋的潜力。采用酶标仪测定Cu2＋对耐辐射球菌的生长速度的影响，用电感耦合等离子光谱法测定菌液离心后得到的上清液Cu2＋浓度。结果表明，当Cu2＋初始质量浓度为1．36mg／L时，耐辐射球菌对其清除效率为57．3％;当-Cu2＋初始质量浓度为6．28mg／L时，对其清除效率为35．4％。此外，用激光共聚焦显微镜观察受到Cu2＋胁迫的耐辐射球菌，发现球菌具有明显的聚集效应。
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| 关 键 词： | 耐辐射球菌Cu2＋ 电感耦合等离子光谱法 去除效率 |
Removal efficiency of Cu2＋ by Deinococcus radiodurans |
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| HAN Wanchun,LI Mingfeng,TIAN Bing,HUA Yuejin,LU Zhenlan.Removal efficiency of Cu2＋ by Deinococcus radiodurans[J].Water Resources Protection,2014(1):64-67. |
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| Authors: | HAN Wanchun LI Mingfeng TIAN Bing HUA Yuejin LU Zhenlan |
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| Institution: | 1. College of Resources and Environment, Jilin Agricultural University, Changchun 130118, China ; 2. Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou 310029, China; 3. Dongying Environmental Protection Agency of Shandong Province, Dongying 257000, China) |
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| Abstract: | As a non-pathogenic bacteria, Deinococcus radiodurans is extremely resistant to ionizing radiation and oxidative stress and shows great potential in remediation of Cu2＋ pollution. In this study, we used the ELIASA plate reader and the ICP-OES, respectively, to monitor the growth rate of Deinococcus radiodurans and the Cu2＋ concentration in the supernatant after the bacterial solution was centrifuged. The results show that the removal efficiency of Cu2＋ by Deinococcus radiodurans reached 57.3% when the initial concentration of Cu2＋ was 1.36mg/L, and the removal efficiency dropped to 35.4% when the initial concentration of Cu2＋ increased to 6.28 mg/L. Meanwhile, using confocal laser scanning microscopy （CLSM）, we observed that Deinococcus radiodurans assembled at a high concentration of Cu2＋. |
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| Keywords: | Deinococcus radiodurans Cu2＋ ICP-OES removal efficiency |
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