| 云芝漆酶的培养和分离纯化的研究 |
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| 引用本文： | 丁少军,宋美静.云芝漆酶的培养和分离纯化的研究[J].纤维素科学与技术,1998,6(3):16-20. |
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| 作者姓名： | 丁少军 宋美静 |
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| 作者单位： | 南京林业大学化工学院!南京，210037，南京林业大学化工学院!南京，210037 |
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| 摘 要： | 研究了云芝009菌株漆酶的培养及分高纯化，其适宜的产酶培养基为Dodson低碳培养基，起始pH值为3.5.同时添加黎芦醇对漆产酶具有强烈的诱导作用．高浓度的Cu2+对产本酶也有促进作用，经过超滤，DEAE－Cellulose和SephadexG－100分离纯化，发现云芝009菌株漆酶由组分A和B组成，其中，B组分占总酶活的85％以上。
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| 关 键 词： | 云芝 漆酶 纯化 |
Study on the production and purification of laccase from Coriolus versicolor |
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| Ding Saojun, Song Meijing.Study on the production and purification of laccase from Coriolus versicolor[J].Journal of Cellulose Science and Technology,1998,6(3):16-20. |
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| Authors: | Ding Saojun Song Meijing |
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| Abstract: | The production and purification of laccase from Coriolus versicolor 009 were studied.The suitab1e medium for its laccase production was Dodson.s low carbon medium with initial pH= 3 .5,The addition of veranyl alcohol great induce the laccase production, high content additionof Cu2 also has some positive effect. After two stage purification by DEAE - celluloce andsephadex G - 100, two fraction laccase A and laccase B were separated. The laccase B consist of85% total laccase activity. |
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| Keywords: | Coriolus versicolor laccase purification |
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