小径纳米纤维素管的生物制备及其表征

以透氧硅胶双套管为模具，设计组装了一套新型生物反应装置，利用红茶菌和套管法生产细菌纤维素（BC）小径人工血管材料。研究发现，细菌纤维素可以分别在内外两根硅胶管的外表面和内表面同时生成，并最终长到一起形成整体BC管。获得的BC管不仅具有较好的强度和光滑平整的内外表面，而且纳米纤维交织紧密，无纤维分层现象，具有突出优点和应用潜力。该制备方法简便易行，成本低廉，生产效率高，可工业化生产。

Biopreparation and Characterization of Small Diameter Nanocellulose Tubes

Development of succedaneous materials for small diameter blood vessels is always a hot but also difficult point in the area of biomedicine. Recently, several technical papers and patents for in situ preparation of small diameter blood vessels by using bacterial cellulose （BC） have been reported. However, the BC tubes that prepared by using the present technology have a drawback, namely having a layered structure that may bring danger after implanting if used as artificial blood vessels. In order to solve this issue, two silicone tubes with different diameter, which have high oxygen permeability, were used as a mould and then a novel bioreactor was constructed for production of BC tubular material as potential artificial blood vessels. Cannulation technology was used to produce BC blood vessels via bacterial fermentation. The bacterial cellulose was simultaneously synthesized both on the inner surface of the outer silicone tube and on the outer surface of the inner silicone tube. Finally, the nano BC fibrils from two direction were interweaved and an integrative bacterial cellulose tubular material was generated. The tubular bacterial cellulose material was analyzed by field emission scanning electron microscopy, and its mechanical properties were determined by tensile strength measurement.