| 稻草纤维素水解机理及工艺条件的研究 |
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| 引用本文： | 吴国杰,柳菲,陈嘉恒,刘美蓉.稻草纤维素水解机理及工艺条件的研究[J].广东化工,2012,39(10):12-13,9. |
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| 作者姓名： | 吴国杰 柳菲 陈嘉恒 刘美蓉 |
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| 作者单位： | 1. 仲恺农业工程学院化学化工学院,广东广州,510225
2. 纽约大学理工学院,纽约布鲁克林11201 |
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| 基金项目： | 广东省自然科学基金项目，仲恺农业工程学院校级重点学科项目 |
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| 摘 要： | 以稻草秸杆为纤维素原料,用2%氢氧化钠对其进行预处理,选用纤维素酶作为催化剂对纤维素进行酶法水解,采用DNS法测定纤维素水解液还原糖的含量,并计算其糖化率。探讨了纤维素酶水解稻草秸秆纤维素的反应机理,研究了纤维素酶的添加量、反应温度、pH、反应时间、振荡等因素对稻草纤维素酶水解的影响。实验结果表明,当固液比为1∶30、纤维素酶添加量为40 mg、50℃、pH 4.8、振荡反应12h,纤维素酶解液的糖化率可达40%。
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| 关 键 词： | 稻草秸秆 纤维素 纤维素酶 水解 糖化率 |
Hydrolysis Mechanism and Technology Condictions of Straw Cellulose |
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| Wu Guojie,Liu Fei,Chen Jiaheng,Liu Meirong.Hydrolysis Mechanism and Technology Condictions of Straw Cellulose[J].Guangdong Chemical Industry,2012,39(10):12-13,9. |
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| Authors: | Wu Guojie Liu Fei Chen Jiaheng Liu Meirong |
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| Affiliation: | 1. School of Chemistry and Chemical Engineering, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China： 2. Polytechnic Institute of New York University, Brooklyn 11201, United States) |
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| Abstract: | Rice straw cellulose was hydrolyzed by cellulase, using 2 % NaOH as pretreatment agent, content of reducing sugar was mensurated using the DNS method, and then the saccharification rate was calculated.The hydrolytic mechanism of straw cellulose was researched, the effect factors such as the adding amount of cellulase, reaction temperature, pH, reaction time, vibration were studied. The experimental results showed that hydrolysis rate could be as high as 40 % as the adding amount of Cellulase was 40 mg, 50 ~C, pH was 4.8, vibration reaction persist with 12h. |
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| Keywords: | rice straw cellulose cellulase hydrolysis saccharificationrate |
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