有机磷农药在菠菜中残留测定方法研究

建立了同时测定菠菜中敌敌畏等6种有机磷农药残留的气相色谱法。结果表明,用丙酮、二氯甲烷提取菠菜中有机磷农药残留,在匀浆、抽滤1次,萃取2次时效果最好,提取的试样经DB-17毛细管柱分离,用配有火焰光度检测器的气相色谱仪能进行准确的定性和定量测定。方法检测下限达6.08×10-4 ~1.6610-3mg/kg,6种有机磷农药的回收率范围为82.52%~92.02%,相对标准偏差(RSD)为6.31%~17.06%。本方法符合国家农药残留量测定方法的要求。

Studies on Residue Determination of Organophosphorus Pesticides in Spinach

An efficient gas chromatographic method for the determination of 6 kinds of organophosphorus pesticide residues in spinach had been established, and the pretreatment of specimen was also discussed. The organophosphorus pesticides were extracted from spinach samples with acetone and dichloromethane. The satisfactory results were obtained when the experiment had been carried out with refining for one time and extraction for two times. The extracted residues were separated through the DB-17 capillary column, then qualitatively and quantitatively measured by gas chromatograph with flame photometric detector. Minimum detection limits of this method were between 6.08×10-4~1.66?0-3mg/kg, the recoveries of organophosphorus pesticide residues ranged from 82.52%~92.02%, with an average RSD of 6.31%~17.06%. The results of this test had met the requirement of national method of pesticide residue analysis.