ENZYME MASS TRANSFER COEFFICIENT IN AQUEOUS TWO PHASE SYSTEMS: SPRAY EXTRACTION COLUMNS |
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Authors: | PA Pa War K Rostami Jafarabad S B Sawant J B Joshi |
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Affiliation: |
a Department of Chemical Technology, University of Bombay, Matunga, Bombay, India |
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Abstract: | Fractional dispersed phase hold-up and overall dispersed phase side mass transfer coefficients were measured in 34, 50, 70 and 95 mm i.d. spray columns using an aqueous two phase system. Sodium sulphate-polyethyleneglycol (PEG) (4000)-water formed the aqueous two phase system and amyloglucosidase was used as a solute for the estimation of mass transfer coefficient.
The dispersed phase hold-up (eD) and volumetric dispersed phase side mass transfer coefficient (KD) both increased with increasing PEG-rich phase velocity. The eDand KDawere found to be independent of the column diameter and column height above a certain critical value. An increase in the phase concentration of sodium sulphate and PEG was found to reduce eDand KDEmpirical and semi-empirical correlations for eD and KDahave been developed. |
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Keywords: | Mass transfer coefficients Spray column Two phase aqueous systems Amyloglucosidase |
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