| 辣根过氧化物酶在三种孔材料上的在线固定化研究 |
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| 引用本文： | 徐芬,王琳,张强,贺业菊,杨梅.辣根过氧化物酶在三种孔材料上的在线固定化研究[J].应用化工,2011,40(5). |
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| 作者姓名： | 徐芬 王琳 张强 贺业菊 杨梅 |
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| 作者单位： | 辽宁师范大学化学化工学院,辽宁大连,116029 |
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| 摘 要： | 利用已建立的顺序注射紫外可见分析系统,以硅基介孔材料MCM-41、SBA-15和炭材料XC-72作为载体,研究和比较了辣根过氧化物酶(HRP)在3种无机孔材料上的在线吸附行为。结果表明,HRP在XC-72上的负载量和结合稳定性高于MCM-41和SBA-15,且以XC-72为载体制备的固定化HRP具有较高的活性和良好的操作稳定性。
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| 关 键 词： | 辣根过氧化物酶 在线固定化 XC-72 MCM-41 SBA-15 |
On-line immobilization of horseradish peroxidase on three porous materials |
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| XU Fen,WANG Lin,ZHANG Qiang,HE Ye-ju,YANG Mei.On-line immobilization of horseradish peroxidase on three porous materials[J].Applied chemical industry,2011,40(5). |
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| Authors: | XU Fen WANG Lin ZHANG Qiang HE Ye-ju YANG Mei |
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| Affiliation: | XU Fen,WANG Lin,ZHANG Qiang,HE Ye-ju,YANG Mei(School of Chemistry and Chemical Engineering,Liaoning Normal University,Dalian 116029,China) |
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| Abstract: | In the present work,mesoporous materials(MCM-41,SBA-15) and carbon(XC-72) were served as supports for the study of on-line immobilization of horseradish peroxidase via a sequential-injection UV-Vis system.The results showed that the binding efficiency and stability of XC-72 for HRP were higher than the other two mesoporous silica materials,and HRP immobilized on XC-72 showed the higher catalytic activity and the better operational stability. |
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| Keywords: | horseradish peroxidase on-line immobilization XC-72 MCM-41 SBA-15 |
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