| 双水相体系酶法合成L-苯丙氨酸的研究 |
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| 引用本文： | 周华,姚忠,孙广海,韦萍.双水相体系酶法合成L-苯丙氨酸的研究[J].化学反应工程与工艺,2006,22(2):146-150. |
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| 作者姓名： | 周华 姚忠 孙广海 韦萍 |
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| 作者单位： | 南京工业大学制药与生命科学学院,江苏,南京,210009 |
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| 基金项目： | 国家研究发展基金;中国科学院资助项目 |
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| 摘 要： | 以天冬氨酸转氨酶为催化剂,开展了双水相体系中苯丙酮酸转氨反应制备L-苯丙氨酸的研究。双水相体系质量组成为聚乙二醇4 000(20%)/Na2HPO4(16%)。苯丙酮酸钠盐、细胞和L-苯丙氨酸在双水相体系中的分配系数分别为8.03,31.7和0.74。当底物浓度为43.4 g/L时,该体系酶法合成L-苯丙氨酸得率为78.1%,比在水溶液体系中提高了27.4%。
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| 关 键 词： | 生物转化 双水相体系 分配系数 L-苯丙氨酸 |
| 文章编号： | 1001-7631(2006)02-0146-05 |
| 收稿时间： | 2005-09-21 |
| 修稿时间： | 2006-01-25 |
Preparation of L-Phenylalanine by an Aqueous Two-Phase System |
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| Zhou Hua,Yao Zhong,Sun Guanghai,Wei Ping.Preparation of L-Phenylalanine by an Aqueous Two-Phase System[J].Chemical Reaction Engineering and Technology,2006,22(2):146-150. |
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| Authors: | Zhou Hua Yao Zhong Sun Guanghai Wei Ping |
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| Affiliation: | College of Pharmacy and Life Science, Nanjing University of Technology, Nanjing 210009, China |
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| Abstract: | The bioconversion of phenylpyruvate to L-phenylalanine using aspartate transaminase in an aqueous two-phase system(ATPS) was investigated.The optimal composition of an aqueous two-phase system was 20% of PEG4000 with 20% of Na_2HPO_4.Partition coefficients of phenylpyruvate,aspartate transaminase and L-phenylalanine respectively were 8.03,31.7 and 0.74 in ATPS.The(enzymatic) yield of L-phenylalanine in ATPS was 78.1%,increased 27.4% higher than in aqueous(system) when the substrate concentration was 43.4 g/L. |
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| Keywords: | bioconversion aqueous two-phase system partition coefficient L-phenylalanine |
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