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放线菌BOS-009的分离、拮抗活性及鉴定
引用本文:王勇,纪明山,张文革,何璐,张丽,梁娇.放线菌BOS-009的分离、拮抗活性及鉴定[J].农药,2009,48(2).
作者姓名:王勇  纪明山  张文革  何璐  张丽  梁娇
作者单位:1. 沈阳农业大学,沈阳,116021辽宁科技大学,辽宁,鞍山,114044
2. 沈阳农业大学,沈阳,116021
3. 辽宁科技大学,辽宁,鞍山,114044
摘    要:对从长白山不同海拔环境中分离筛选出可以产生抗菌活性物质的BOS-009放线菌菌株进行了研究,测定了该菌的抗菌谱,发现该菌株对多种植物病原性真菌及细菌有拮抗作用.通过形态特征、培养特征观察,生理生化特性及抑菌谱等系列比较.发现该菌株与链霉菌属的除虫链霉菌(Streptomyces avermitilis)的特征基本相符;通过16s rDAN序列比对,发现该菌株16s rDAN与除虫链霉菌的16s rDAN同源性达99%.根据多项分类原则和系统进化树的构建分析,将该菌株暂归入除虫链霉菌类.

关 键 词:放线菌  拮抗  植物病原菌  鉴定

Separating, Antagonistic Activity and Identifying of Actinomycetes(BOS-009)
WANG Yong,JI Ming-shan,ZHANG Wen-ge,HE Lu,ZHANG Li,LIANG Jiao.Separating, Antagonistic Activity and Identifying of Actinomycetes(BOS-009)[J].Pesticides,2009,48(2).
Authors:WANG Yong  JI Ming-shan  ZHANG Wen-ge  HE Lu  ZHANG Li  LIANG Jiao
Affiliation:WANG Yong1,2,JI Ming-shan1,ZHANG Wen-ge3,HE Lu2,ZHANG Li2,LIANG Jiao2
Abstract:A strain of actinomycetes BOS-009 was obtained by separating difference soil or water samples of areas from Changbai mountain. Inhition spectrum test showed that BOS-009 strain had antagonistic activity to fungus and bacteria. Moreover, the morphology, culture characteristics, physiological-biochemical characteristics of BOS-009 was similar to Streptomyces avermitilis, and the homology were 99% through compared its 16s rDAN. BOS-009 strain was identified as Streptomyces avermitilis according to analyze prin...
Keywords:actinomycetes  antagonism  plant pathogeny  identification  
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