Fabrication and characterization of poly(ε-caprolactone)/gelatin nanofibrous scaffolds for retinal tissue engineering |
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| Authors: | Shiva Rahmani Shahab Faghihi Ghassem Amoabediny Mehdi Shakibaie Behnam Noorani |
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| Affiliation: | 1. Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran;2. Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran;3. Tissue Engineering and Biomaterials Research Center, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran;4. Department of Chemical Engineering, Faculty of Engineering, University of Tehran, Tehran, Iran;5. Research Center for New Technologies in Life Science Engineering, University of Tehran, Tehran, Iran |
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| Abstract: | Different ratios of poly(ε-caprolactone) (PCL) and gelatinwere used to fabricate scaffolds for regeneration of retinal pigment epithelium (RPE) layer. Physical and chemical characterizations were performed and the behavior of human RPE cells on the scaffolds was evaluated subsequently. An increase in gelatin content in the scaffold enhanced hydrophilicity, RPE cell attachment, proliferation, and spreading over PCL scaffolds. Granular and cytoplasmic expressions of RPE65 and Cytokeratin 8/18 markers confirmed the presence of RPE cells. It was believed that PCL/gelatin scaffolds could be used as substrates to replace RPE extracellular matrix to facilitate regeneration of RPE layer in retinal diseases. |
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| Keywords: | Electrospinning gelatin nanofibrous scaffolds poly(ε-caprolactone) retinal pigment epithelium retinal tissue engineering |
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