Virus-Based Nanoreactors with GALT Activity for Classic Galactosemia Therapy |
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Authors: | Pedro Gama Dr Ruben D Cadena-Nava Dr Karla Juarez-Moreno Dr Javier Pérez-Robles Prof Rafael Vazquez-Duhalt |
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Affiliation: | Department of Bionanotechnology, Centro de Nanociencias y Nanotecnología, Universidad Nacional Autónoma de México, Ensenada, Baja California, Mexico |
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Abstract: | Enzymatic nanoreactors were obtained by galactose-1-phosphate uridylyl-transferase (GALT) encapsulation into plant virus capsids by a molecular self-assembly strategy. The aim of this work was to produce virus-like nanoparticles containing GALT for an enzyme-replacement therapy for classic galactosemia. The encapsulation efficiency and the catalytic constants of bio-nanoreactors were determined by using different GALT and virus coat protein ratios. The substrate affinity of nanoreactors was slightly lower than that of the free enzyme; the activity rate was 16 % of the GALT free enzyme. The enzymatic nanoreactors without functionalization were internalized into different cell lines including fibroblast and kidney cells, but especially into hepatocytes. The enzymatic nanoreactors are an innovative enzyme preparation with potential use for the treatment of classic galactosemia. |
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Keywords: | bio-nanoreactor classic galactosemia galactose-1-phosphate uridylyl-transferase GALT virus-like nanoparticles |
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