HPLC法同时测定发酵液中纽莫康定Ao与Bo的含量

采用HPLC方法同时测定发酵液中纽莫康定A。与＆的含量。色谱柱为SepaxSapphireC，8（4．6mm×250rrlm，5“m），流动相为甲醇一水（体积比45：55，内含0．1‰三氟乙酸），流速为1．OmL·min＾－1，紫外检测波长为214rim。结果表明，纽莫康定A。与B0的浓度分别在47．5～191．4pg·mL。和38．4～153．6肛g·mL-1的范围内，与峰面积呈良好的线性关系（R分别为0．9997和0．9998）；检测灵敏度分别为6．86ng、5．95ng；平均回收率分别为99．15％（RSD一0．96％，n一9）和99．31％（RSD=0．98％，n一9）。该方法简便、准确、重现性好，可用于发酵液中纽莫康定A。与B0含量的测定。

Simultaneous Determination of Content of Pneumocandin Ao and Bo in Fermentation Broth by HPLC

Simultaneous determination of content of pneumocandin A0 and B0 in fermentation broth by HPLC was studied. The HPLC conditions consisted of a SepaxSapphire C18 column （4.6 ram）〈 250 mm,5 μm）,mobile phase of methanol-H20 （45 ： 55） containing 0.1%0 trifluoracetic acid with a flow rate of 1.0 mL ·min-1 and detection wavelength at 214 nm. The linearity of pneumocandin A0 and B0 concentration was shown in the range of 47.5-191.4 /,g ~ mL-1 （R：0. 9997） and 38.4-153.6 /,g · mL-1 （R=0. 9998） ;the sensitivity of pneumocandin Ao and B0 was 6.86 ng, 5.95 ng;the average recovery of pneumocandin Ao and Bo was 99.15~//00 with RSD =0.96%（n=9） and 99.31% with RSD=0. 980% （n=9）,respectively. The HPLC method, which was simple and accurate with good repeatability, could be applied to simultaneous determination of content of pneumocan- din Ao and Bo in fermentation broth.