Controlled Production of Amyloid β Peptide from a Photo‐Triggered,Water‐Soluble Precursor “Click Peptide“ |
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Authors: | Atsuhiko Taniguchi Mariusz Skwarczynski Dr Youhei Sohma Dr Takuma Okada Dr Keisuke Ikeda Halan Prakash Dr Hidehito Mukai Dr Yoshio Hayashi Prof Dr Tooru Kimura Dr Shun Hirota Prof Dr Katsumi Matsuzaki Prof Dr Yoshiaki Kiso Prof Dr |
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Affiliation: | 1. Department of Medicinal Chemistry, Center for Frontier Research in Medicinal Science, 21st Century COE Program, Kyoto Pharmaceutical University, Yamashina-ku, Kyoto 607-8412 (Japan), Fax: (+81) 75-591-9900;2. Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-ku, Kyoto 606-8501 (Japan);3. Graduate School of Materials Science, Nara Institute of Science and Technology, Nara 630-0192 (Japan);4. Department of Medicinal Chemistry, Center for Frontier Research in Medicinal Science, 21st Century COE Program, Kyoto Pharmaceutical University, Yamashina-ku, Kyoto 607-8412 (Japan), Fax: (+81) 75-591-9900
School of Pharmacy, Tokyo University of Pharmacy and Life Science, Tokyo 192-0392 (Japan) |
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Abstract: | In biological experiments, poor solubility and uncontrolled assembly of amyloid β peptide (Aβ) 1–42 pose significant obstacles to establish an experiment system that clarifies the function of Aβ1–42 in Alzheimer's disease (AD). Herein, as an experimental tool to overcome these problems, we developed a water‐soluble photo‐“click peptide” with a coumarin‐derived photocleavable protective group that is based on an O‐acyl isopeptide method. The click peptide had nearly 100‐fold higher water solubility than Aβ1–42 and did not self‐assemble, as the isomerized structure in its peptide backbone drastically changed the conformation that was derived from Aβ1–42. Moreover, the click peptide afforded Aβ1–42 quickly under physiological conditions (pH 7.4, 37 °C) by photoirradiation followed by an O–N intramolecular acyl migration. Because the in situ production of intact Aβ1–42 from the click peptide could improve the difficulties in handling Aβ1–42 caused by its poor solubility and highly aggregative nature, this click peptide strategy would provide a reliable experiment system for investigating the pathological function of Aβ1–42 in AD. |
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Keywords: | Alzheimer's disease amyloid‐beta peptides click peptides O‐acyl isopeptide method photolysis |
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