1. Department of Human and Animal Biology, University of Torino, Via Accademia Albertina 13, 10123 Torino (Italy), Fax: (+39)?011‐6704508;2. Department of Public Health and Microbiology, University of Torino, Via Santena 9, 10126 Torino (Italy)
Abstract:
Choosing chloro : By reshaping the catalytic pocket of a catechol 1,2‐dioxygenase through a structural route alternative to evolution, novel engineered chlorocatechol dioxygenase‐like enzymes were obtained. Variants show an inversion of specificity with a preference for 4‐chlorocatechol and activity on the rarely recognised substrate 4,5‐dichlorocatechol.
