Gastric Adenocarcinomas and Signet-Ring Cell Carcinoma: Unraveling Gastric Cancer Complexity through Microbiome Analysis—Deepening Heterogeneity for a Personalized Therapy |
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| Authors: | Gloria Ravegnini Bruno Fosso Viola Di Saverio Giulia Sammarini Federica Zanotti Giulio Rossi Monica Ricci Federica D&#x;Amico Giorgia Valori Antonella Ioli Silvia Turroni Patrizia Brigidi Patrizia Hrelia Sabrina Angelini |
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| Affiliation: | 1.Department of Pharmacy and Biotechnology, University of Bologna, 40126 Bologna, Italy; (G.R.); (G.S.); (F.Z.); (F.D.); (G.V.); (S.T.); (P.H.);2.National Research Council, Institute of Biomembranes, Bioenergetics and Molecular Biotechnologies (IBIOM), 70126 Bari, Italy;3.Anatomy and Pathological Histology Unit, Infermi Hospital, 47923 Rimini, Italy; (V.D.S.); (G.R.); (M.R.); (A.I.);4.Department of Medical and Surgical Sciences, University of Bologna, 40126 Bologna, Italy; |
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| Abstract: | Gastric cancer (GC) is the fifth most prevalent cancer worldwide and the third leading cause of global cancer mortality. With the advances of the omic studies, a heterogeneous GC landscape has been revealed, with significant molecular diversity. Given the multifaceted nature of GC, identification of different patient subsets with prognostic and/or predictive outcomes is a key aspect to allow tailoring of specific treatments. Recently, the involvement of the microbiota in gastric carcinogenesis has been described. To deepen this aspect, we compared microbiota composition in signet-ring cell carcinoma (SRCC) and adenocarcinoma (ADC), two distinct GC subtypes. To this purpose, 10 ADC and 10 SRCC and their paired non-tumor (PNT) counterparts were evaluated for microbiota composition through 16S rRNA analysis. Weighted and unweighted UniFrac and Bray–Curtis dissimilarity showed significant community-level separation between ADC and SRCC. Through the LEfSe (linear discriminant analysis coupled with effect size) tool, we identified potential microbial biomarkers associated with GC subtypes. In particular, SRCCs were significantly enriched in the phyla Fusobacteria, Bacteroidetes, Patescibacteria, whereas in the ADC type, Proteobacteria and Acidobacteria phyla were found. Overall, our data add new insights into GC heterogeneity and may contribute to deepening the GC classification. |
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| Keywords: | gastric cancer adenocarcinoma ADC signet-ring cell carcinoma SRCC microbiome biomarkers personalized therapy |
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