Skullcapflavone II Suppresses TNF-α/IFN-γ-Induced TARC,MDC, and CTSS Production in HaCaT Cells |
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Authors: | Hanon Lee Dong Hun Lee Jang-Hee Oh Jin Ho Chung |
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Affiliation: | 1.Department of Biomedical Sciences, Seoul National University Graduate School, Seoul 03080, Korea;2.Department of Dermatology, Seoul National University College of Medicine, Seoul 03080, Korea;3.Institute of Human-Environment Interface Biology, Medical Research Center, Seoul National University, Seoul 03080, Korea;4.Laboratory of Cutaneous Aging Research, Biomedical Research Institute, Seoul National University Hospital, Seoul 03080, Korea |
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Abstract: | Skullcapflavone II (SFII), a flavonoid derived from Scutellaria baicalensis, has been reported to have anti-inflammatory properties. However, its therapeutic potential for skin inflammatory diseases and its mechanism are unknown. Therefore, this study aimed to investigate the effect of SFII on TNF-α/IFN-γ-induced atopic dermatitis (AD)-associated cytokines, such as thymus- and activation-regulated chemokine (TARC) and macrophage-derived chemokine (MDC). Co-stimulation with TNF-α/IFN-γ in HaCaT cells is a well-established model for induction of pro-inflammatory cytokines. We treated cells with SFII prior to TNF-α/IFN-γ-stimulation and confirmed that it significantly inhibited TARC and MDC expression at the mRNA and protein levels. Additionally, SFII also inhibited the expression of cathepsin S (CTSS), which is associated with itching in patients with AD. Using specific inhibitors, we demonstrated that STAT1, NF-κB, and p38 MAPK mediate TNF-α/IFN-γ-induced TARC and MDC, as well as CTSS expression. Finally, we confirmed that SFII significantly suppressed TNF-α/IFN-γ-induced phosphorylation of STAT1, NF-κB, and p38 MAPK. Taken together, our study indicates that SFII inhibits TNF-α/IFN-γ-induced TARC, MDC, and CTSS expression by regulating STAT1, NF-κB, and p38 MAPK signaling pathways. |
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Keywords: | Skullcapflavone II TARC MDC CTSS STAT1 NF-κ B p38 MAPK anti-inflammatory activity atopic dermatitis keratinocytes |
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