Characterization of Dog Glutathione Transferase P1-1, an Enzyme Relevant to Veterinary Medicine |
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| Authors: | Aram Ismail Elizabeth Lewis Birgitta Sjdin Bengt Mannervik |
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| Affiliation: | 1.Department of Biochemistry and Biophysics, Arrhenius Laboratories, Stockholm University, SE-10691 Stockholm, Sweden; (A.I.); (B.S.);2.College of Liberal Arts & Sciences, University of Illinois Urbana-Champaign, Champaign, IL 61801, USA; |
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| Abstract: | Glutathione transferases (GSTs) form a family of detoxication enzymes instrumental in the inactivation and elimination of electrophilic mutagenic and carcinogenic compounds. The Pi class GST P1-1 is present in most tissues and is commonly overexpressed in neoplastic cells. GST P1-1 in the dog, Canis lupus familiaris, has merits as a marker for tumors and as a target for enzyme-activated prodrugs. We produced the canine enzyme CluGST P1-1 by heterologous bacterial expression and verified its cross-reactivity with antihuman-GST P1-1 antibodies. The catalytic activity with alternative substrates of biological significance was determined, and the most active substrate found was benzyl isothiocyanate. Among established GST inhibitors, Cibacron Blue showed positive cooperativity with an IC50 value of 43 nM. Dog GST P1-1 catalyzes activation of the prodrug Telcyta, but the activity is significantly lower than that of the human homolog. |
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| Keywords: | Telcyta veterinary medicine enzyme-activated chemotherapy prodrugs dog GST P1-1 |
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