Identification of Specific Variations in a Non-Motile Strain of Cyanobacterium Synechocystis sp. PCC 6803 Originated from ATCC 27184 by Whole Genome Resequencing |
| |
Authors: | Qinglong Ding Gu Chen Yuling Wang Dong Wei |
| |
Affiliation: | College of Light Industry and Food Sciences, South China University of Technology, 381 Wushan Road, 510641 Guangzhou, China; E-Mails: (Q.D.); (Y.W.); (D.W.) |
| |
Abstract: | Cyanobacterium Synechocystis sp. PCC 6803 is a widely used model organism in basic research and biofuel biotechnology application. Here, we report the genomic sequence of chromosome and seven plasmids of a glucose-tolerant, non-motile strain originated from ATCC 27184, GT-G, in use at Guangzhou. Through high-throughput genome re-sequencing and verification by Sanger sequencing, eight novel variants were identified in its chromosome and plasmids. The eight novel variants, especially the five non-silent mutations might have interesting effects on the phenotype of GT-G strains, for example the truncated Sll1895 and Slr0322 protein. These resequencing data provide background information for further research and application based on the GT-G strain and also provide evidence to study the evolution and divergence of Synechocystis 6803 globally. |
| |
Keywords: | Synechocystis sp PCC 6803 whole genome resequencing single nucleotide polymorphism deletion insertion |
|
|