Production of specific-structured lipids by enzymatic interesterification: Elucidation of Acyl migration by response surface design |
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Authors: | X Xu A R H Skands C -E Høy H Mu S Balchen J Adler-Nissen |
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Affiliation: | (1) Department of Biochemistry & Nutrition, Technical University of Denmark, DK-2800 Lyngby, Denmark;(2) Department of Biotechnology, Technical University of Denmark, Building 221, DK-2800 Lyngby, Denmark |
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Abstract: | Production of specific-structured lipids (SSL) by lipase-catalyzed interesterification has been attracting more and more attention
recently. However, it was found that acyl migration occurs during the reaction and causes the production of by-products. In
this paper, the elucidation of acyl migration by response surface design was carried out in the Lipozyme IM (Rhizomucor miehei)-catalyzed interesterification between rapeseed oil and capric acid in solvent-free media. A five-factor response surface
design was used to evaluate the influence of five major factors and their relationships. The five factors, water content,
reaction temperature, enzyme load, reaction time and substrate ratio, were varied at three levels together with two star points.
All parameters besides substrate ratio had strong positive influences on acyl migration, and reaction temperature was most
significant. The contour plots clearly show the interactions between the parameters. The migration rates of different fatty
acids were also compared from three different sets of experiments during the lipase-catalyzed reaction. The best-fitting quadratic
response surface model was determined by regression and backward elimination. The coefficients of determination (R
2) of the model were 0.996 and 0.981 for Q
2 value. The results show that the fitted quadratic model satisfactorily expresses acyl migration for the enzymatic interesterification
in the batch reactor used. |
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Keywords: | Acyl migration fish oil lipase-catalyzed interesterification rapeseed oil response surface methodology Rhizomucor miehei specific-structured lipids |
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