Enzymatic Production of Monoacylglycerols with Camellia Oil by the Glycerolysis Reaction |
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Authors: | Fan-kui Zeng Bo Yang Yong-hua Wang Wei-fei Wang Zheng-xiang Ning Lin Li |
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Affiliation: | (1) College of Light Industry and Food Sciences, South China University of Technology, 510640 Guangzhou, People’s Republic of China;(2) School of Bioscience and Bioengineering, South China University of Technology, 510006 Guangzhou, People’s Republic of China; |
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Abstract: | Three commercial immobilized lipases, Lipozyme RM IM, Lipozyme TL IM and Novozym 435, were screened for the production of
monoacylglycerols (MAG) by glycerolysis of camellia oil in a solvent medium of tert-butyl alcohol. Novozym 435 showed the best performance and was selected to catalyze the glycerolysis reaction. Different
reaction conditions for the batch reaction, substrate mole ratio, substrate concentration and temperature, were investigated.
The optimal reaction conditions were determined as 6:1 mole ratio of glycerol to camellia oil at 40% (w/v) of substrate concentration
in tert-butyl alcohol at a reaction temperature of 50 °C. Under these optimal conditions, the conversion rate of camellia oil was
98.7% (10 h), and the mixture of acylglycerols contained 82.0% of MAG. A packed-bed reactor (PBR) system with 4.5 g Novozym
435 was employed in continuous production. The resulting product mixture of acylglycerols contained 80.74% of MAG and was
obtained at a flow rate of 0.25 mL/min of substrates. The long-term operation of the PBR system gave an average productivity
of 0.698 kg MAG/(kg enzyme h) after 38 days of operation. |
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