| 重组人白细胞介素-31表达条件的优化 |
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| 引用本文： | 余妍,孙万邦,黄俊琼,曹雪梅,郑静,魏培.重组人白细胞介素-31表达条件的优化[J].粉末涂料与涂装,2008,21(9). |
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| 作者姓名： | 余妍 孙万邦 黄俊琼 曹雪梅 郑静 魏培 |
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| 作者单位： | 遵义医学院珠海校区免疫学教研室 |
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| 摘 要： | 目的优化重组人白细胞介素-31(rhIL-31)的表达条件。方法以不同浓度IPTG、不同诱导时间、不同诱导温度对含有pET32a/rhIL-31的工程菌E.coliBL21(DE3)进行诱导表达,用SDS-PAGE对表达产物进行分析。结果诱导时间为5h,IPTG浓度为1.5mmol/L,诱导温度为37℃时,rhIL-31的表达量最高。结论已初步优化了重组蛋白rhIL-31的表达条件。
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| 关 键 词： | 人白细胞介素-31 重组蛋白 表达条件 优化 |
Optimization of Condition for Expression of Recombinant Human Interleukin-31 |
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| YU Yan,SUN Wan-bang,HUANG Jun-qiong,et al.Optimization of Condition for Expression of Recombinant Human Interleukin-31[J].Chinese Journal of Biologicals,2008,21(9). |
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| Authors: | YU Yan SUN Wan-bang HUANG Jun-qiong |
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| Abstract: | Objective To optimize the condition for expression of recombinant human interleukin-31(rhIL-31). Methods Recombinant E. coli with plasmid pET32a / rhIL-31 was induced at various temperatures with IPTG at different concentrations for different hours, and the expressed protein was identified by SDS-PAGE. Results The expression level of rhIL-31 reached a peak value after the recombinant E. coli was induced with 1. 5 mmol / L IPTG at 37℃ for 5 h. Conclusion The condition for expression of rhIL-31 was preliminarily optimized. |
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| Keywords: | Human interleukin-31 Recombinant protein Expression condition Optimization |
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