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| H_2O_2对Apg-2与BCR/ABL蛋白亚细胞定位的影响 | |
| 引用本文： | 李春莉,刘钉宾,袁颖,陶崑,史梦,冯文莉. H_2O_2对Apg-2与BCR/ABL蛋白亚细胞定位的影响[J]. 中国生物制品学杂志, 2012, 25(11): 1482-1484 |
| 作者姓名： | 李春莉 刘钉宾 袁颖 陶崑 史梦 冯文莉 |
| 作者单位： | 1. 重庆市妇幼保健院检验科,重庆,400013 2. 重庆医科大学临床血液学教研室临床检验诊断学教育部重点实验室,重庆,400016 3. 重庆医科大学附一院普外科,重庆,400016 |
| 基金项目： | 教育部博士学科点专项基金(项目编号：2005631007) |
| 摘 要： | 目的观察H2O2对Apg-2与BCR/ABL蛋白亚细胞定位的影响。方法用50μmol/L H2O2分别处理BaF3-BCR/ABL、BaF3-MIGR1及过表达Apg-2蛋白的BaF3-BCR/ABL细胞,激光共聚焦显微镜观察细胞内Apg-2、BCR/ABL蛋白的亚细胞定位变化。结果 Apg-2和BCR/ABL蛋白在细胞中定位于胞浆,H2O2损伤可致Apg-2和BCR/ABL蛋白在BaF3-BCR/ABL细胞中发生核转位,Apg-2蛋白过表达可引起BCR/ABL蛋白核转位。结论 Apg-2蛋白在H2O2诱导的损伤后发生核转位,可能与BCR/ABL蛋白相互作用,保护H2O2诱导的细胞损伤。 |
| 关 键 词： | Apg-2蛋白 BCR/ABL蛋白 过氧化氢 亚细胞定位 |
Effect of H2O2 on subcellular localization of Apg-2 and BCR/ABL proteins |
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| Abstract: | Objective To observe the effect of H2O2 on subcellular localization of Apg-2 and BCR / ABL proteins.Methods BaF3-BCR / ABL and BaF3-MIGR1 cells as well as BaF3-BCR / ABL cells in which Apg-2 was overexpressed were treated with 50 μmol / L H2O2 respectively,and determined for subcellular localization of Apg-2 and BCR / ABL proteins by confocal microscopy.Results Both Apg-2 and BCR / ABL proteins were located in cytoplasm.However,H2O2 treatment caused the translocation of Apg-2 and BCR / ABL proteins,while the overexpression of Apg-2 caused the translocation of BCR / ABL,into nucleus of BaF3-BCR / ABL cells.Conclusion H2O2 induced-damage contributed to nuclear translocation of Apg-2 which might interact with BCR / ABL to protect cells from the damage. |
| Keywords: | Apg-2 protein BCR/ABL protein Hydrogen peroxide Subcellular localization |
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