轮状病毒P[2]G3株在Vero细胞上的传代适应性

目的研究轮状病毒P2]G3株在Vero细胞上的传代适应性,以确定其在Vero细胞上培养的最适条件。方法将P2]G3株轮状病毒分别以0.01、0.05和0.1MOI接种于Vero细胞,观察细胞病变(CPE)情况,并检测病毒的感染性滴度,确定在Vero细胞上接种病毒的最适MOI。同时观察连续传15代后,各代次病毒的滴度及其基因核酸带型的变化。结果MOI为0.05时,接种病毒后出现CPE及收获时间均较早,病毒的感染性滴度最高,确定接种病毒的最适MOI为0.05。连续传15代,病毒滴度随传代次数的增加而上升,各代次病毒的基因组核酸带型基本一致,且与原代病毒相符。结论轮状病毒P2]G3株经适应性培养后,可在Vero细胞上稳定传代15代。

Adaptability of Rotavirus P[2]G3 Strain in Vero Cells

Objective To study the adaptability of rotavirus P2]G3 strain in Vero cells and optimize the condition for cul-ture of the strain in Vero cells.Methods Rotavirus P2]G3 strain was inoculated into Vero cells at MOIs of 0.01,0.05 and 0.1 respectively,then the CPE was observed and the infectious titer of virus was determined,based on which the MOI was optimized.The virus strain was subcultured in Vero cells for 15 passages,and each passage was determined for titer and observed for genome banding pattern.Results The optimal MOI for inoculation of rotavirus P2]G3 strain into Vero cells was 0.05.During subculture,the virus titer increased with the increasing passage,and the genome banding pattern of various passages were basically consistent with that of original strain.Conclusion The rotavirus P2]G3 strain after adaptation may be subcultured stably for 15 passages in Vero cells.