| 用硫酸鱼精蛋白去除Vero细胞乙型脑炎疫苗中残余DNA |
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| 引用本文： | 石慧颖,丁志芬.用硫酸鱼精蛋白去除Vero细胞乙型脑炎疫苗中残余DNA[J].粉末涂料与涂装,1997,10(3):169-171. |
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| 作者姓名： | 石慧颖 丁志芬 |
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| 作者单位： | 卫生部北京生物制品研究所!北京，100024，卫生部北京生物制品研究所!北京，100024，卫生部北京生物制品研究所!北京，100024，卫生部北京生物制品研究所!北京，100024，卫生部北京生物制品研究所!北京，100024 |
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| 摘 要： | 通过试验研究建立一种去除Vero细胞乙脑疫苗中残余细胞DNA的方法，即疫苗经超滤浓缩后加入0.8～1.0mg／ml的硫酸色精蛋白，于4℃静置2h，8000r／min离心5min。DNA与鱼精蛋白共同沉于管底。用该法处理的疫苗不仅使残余DNA达到规程要求（＜100pg／剂量），而且抗原活性无明显丢失。
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| 关 键 词： | 乙型脑炎疫苗 细胞基质DNA 硫酸鱼精蛋白 |
Removement of Residual Cellular DNA in JE Vaccine Prepared with Vero Cells by Protamine Sulfate |
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| Shi huiying, Ding Zhifen, Li Jing et al.Removement of Residual Cellular DNA in JE Vaccine Prepared with Vero Cells by Protamine Sulfate[J].Chinese Journal of Biologicals,1997,10(3):169-171. |
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| Authors: | Shi huiying Ding Zhifen Li Jing |
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| Abstract: | A method for removing residual cellular DNA in JE vaccine prepared with Vero cells was developed. The vaccine after being concentrated by ultrafiltration was added with 0. 8 ~ 1.0mg/ml protamine sulfate and stored at 4℃ for 2h before centrifugation (10000g/min, 5min). The result showed that cellular DNA was precipitated at the bottom ofcentrifuge tube along with the protamine sulfate. The residual DNA content in the vaccine treated by the method could meet the Chinese Requirements for Bilolgicals (<100pg/dose). Itwas found no significant loss of antigenic activity. |
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| Keywords: | JE vaccine Residual cellular DNA Protamine sulfate |
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