3株新城疫病毒体外对人喉癌Hep-2细胞杀伤效应的比较

目的比较新城疫病毒(NDV)强毒株D817、弱毒株7793和La Sota疫苗株对人喉癌Hep-2细胞的杀伤效应。方法3株病毒经扩增、噬斑纯化后,分别感染Hep-2细胞和喉正常组织细胞,一定时间后,显微镜下观察细胞形态变化,MTT比色法检测病毒对细胞的杀伤效应。结果3株病毒均能在Hep-2细胞中增殖并杀伤细胞,杀伤效应以D817株最高,La Sota株其次,7793株最低,且差异均无统计学意义。杀伤效应与病毒剂量和作用时间呈正相关,显微镜下可见明显的细胞病变。而对喉正常组织细胞的杀伤效应不明显。结论NDV D817株和7793株对喉癌细胞的杀伤效应与La Sota疫苗株相似,而对喉正常组织细胞的杀伤效应不明显,有望成为喉癌生物学治疗新的候选病毒株。

In Vitro Killing Effects of 3 Newcastle Disease Virus Strains on Human Throat Cancer Hep-2 Cells

Objective To compare the in vitro killing effects of 3 Newcastle disease virus(NDV)strains on human throat cancer Hep-2 cells.Methods The virulent D817,attenuated 7793 and La Sota strains of NDV were proliferated and purified,then used for infection of Hep-2 cells respectively,using normal human throat tissue cells as control.The morphological change of Hep-2 cells was observed by microscopy,and the killing effect was determined by MTT method.Results All the 3 virus strains were prolif-erated in Hep-2 cells and killed the cells,while showed no significantly killing effect on normal throat tissue cells.In the turn of killing effect on Hep-2 cells,the 3 strains were D817,La Sota and 7793.However,no significant differences were observed between killing effects of the 3 strains.The killing effect was positively related to the dosage of virus and time for treatment.Obvious CPE of Hep-2 cells was observed by microscopy.Conclusion The killing effects of D817 and 7793 strains of NDV on Hep-2 cells were e-quivalent to that of La Sota strain.However,neither of the strain showed significantly killing effect on normal throat tissue cells,indi-cating that D817 and 7793 strains might be used as novel candidate virus strains for biotherapy of throat cancer.