| 蛋白对HCV/HBV DNA疫苗的免疫增强作用 |
| |
| 引用本文： | 马绍辉,刘龙丁,李健峰,董承红,胡方,陈丽珊,任大明,李琦涵.蛋白对HCV/HBV DNA疫苗的免疫增强作用[J].粉末涂料与涂装,2006,19(1):49-51. |
| |
| 作者姓名： | 马绍辉 刘龙丁 李健峰 董承红 胡方 陈丽珊 任大明 李琦涵 |
| |
| 作者单位： | 中国医学科学院中国协和医科大学医学生物学研究所 昆明650118(马绍辉，刘龙丁，李健峰，董承红，胡方，李琦涵)，上海复旦大学遗传所 上海200433(陈丽珊，任大明) |
| |
| 基金项目： | 云南省科委省院省校合作基金资助(200YF01) |
| |
| 摘 要： | 目的探讨蛋白对HCV/HBV DNA疫苗的免疫增强作用。方法将构建的HCV/HBV真核表达载体pcDNA-PCXS和蛋白分别免疫BALB/c小鼠,用ELISA的方法检测抗-HBs和抗-HCV Ab;3H-TdR掺入法检测免疫小鼠T淋巴细胞增殖;51Cr释放法检测免疫小鼠特异性CTLs杀伤作用。结果DNA-蛋白组抗-HBs和抗-HCV抗体均较DNA组出现早,且抗体水平明显高,DNA-蛋白组的CTL应答水平也明显高于DNA组。结论蛋白能提高DNA疫苗的特异性体液和细胞免疫功能,为DNA疫苗的实际应用提供了一种新的策略。
|
| 关 键 词： | 乙型肝炎病毒 丙型肝炎病毒 DNA-蛋白免疫 免疫反应 |
| 收稿时间： | 2005-05-18 |
| 修稿时间： | 2005年1月26日 |
Immunogenicity of Combined HCV/HBV Immunization by DNA Prime-Protein Boost Strategy |
| |
| MA Shao-hui , LIU Long-ding, LI Jian-feng, et al.Immunogenicity of Combined HCV/HBV Immunization by DNA Prime-Protein Boost Strategy[J].Chinese Journal of Biologicals,2006,19(1):49-51. |
| |
| Authors: | MA Shao-hui LIU Long-ding LI Jian-feng |
| |
| Affiliation: | Institute of Medical Biology, Chinese Academy of Medical Sciences ,Peking Union Medical College,Kunming 650118, China |
| |
| Abstract: | Objective To explore the effect of hepatitis C virus(HCV)/hepatitis B virus(HBV) bivalent vaccine by a prime-boost strategy.Methods Immunize BALB/c mice with the constructed eukaryotic expression vectors pcDNA-PCXS,pcDNA-PCSS S/PCX protein and pcDNA3.0 respectively.Determine anti-HBs and anti-HCV antibodies by ELISA,T lymphocyte proliferation by ~3H-TdR incorporation and specific killing activity of CTLs by ~ 51 Cr release assay.Results Compared with those immunized with pcDNA-PCXS,high anti-HBs and anti-HCV titers were induced early in mice immunized with pcDNA-PCXS S/PCX protein.The specific CTL response level induced by pcDNA-PCXS S/PCX protein was significantly higher than that induced by pcDNA-PCXS.Conclusion DNA prime-protein boost immunization enhanced the humoral and cellular immunological functions of immunized mice.It provided a novel strategy for the practical application of DNA vaccine. |
| |
| Keywords: | Hepatitis B virus Hepatitis C virus DNA prime-boost immunization Immune response |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
