| BCG-CpG-DNA对重组HBsAg的免疫佐剂作用 |
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| 引用本文： | 赵爱华,乔来艳,贾淑珍,寇丽杰,王国治.BCG-CpG-DNA对重组HBsAg的免疫佐剂作用[J].粉末涂料与涂装,2007,20(5):356-358,361. |
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| 作者姓名： | 赵爱华 乔来艳 贾淑珍 寇丽杰 王国治 |
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| 作者单位： | 中国药品生物制品检定所菌苗室 (北京100050) |
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| 摘 要： | 目的研究BCG-CpG-DNA对重组HBsAg的免疫佐剂作用。方法以BCG-CpG-DNA与重组HBsAg混合免疫小鼠,以铝佐剂疫苗为对照,采用放射免疫法检测抗-HBs中和抗体水平,ELISA法分析抗体亚类,酶联免疫斑点试验(ELIS-POT)检测CD8+细胞毒T淋巴细胞(CTL)功能。结果BCG-CpG-DNA能提高抗-HBs中和抗体水平,促进抗原特异性IgG2a的产生,诱导Th1型免疫应答,与铝佐剂协同作用能部分逆转Th2反应。同时能增强抗原特异性CD8+T淋巴细胞的杀伤作用,实验组与对照组比较差异有显著意义。结论BCG-CpG-DNA对HBsAg有较好的免疫佐剂作用,有可能成为一种新型的免疫佐剂。
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| 关 键 词： | BCG-CpG-DNA 免疫佐剂 HBsAg |
| 文章编号： | 1004-5503（2007）05-356-04 |
| 收稿时间： | 2006-08-18 |
| 修稿时间： | 2006-08-18 |
Effect of BCG-CpG-DNA as An Immunoadjuvant of Recombinant HBsAg |
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| ZHAO Ai-hua, QIAO Lai-yan, JIA Shu-zhen, et al.Effect of BCG-CpG-DNA as An Immunoadjuvant of Recombinant HBsAg[J].Chinese Journal of Biologicals,2007,20(5):356-358,361. |
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| Authors: | ZHAO Ai-hua QIAO Lai-yan JIA Shu-zhen |
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| Affiliation: | National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China |
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| Abstract: | Objective To study the effect of BCG-CpG-DNA as an immunoadjuvant of recombinant HBsAg.Methods Immunize mice with the mixture of BCG-CpG-DNA and recombinant HBsAg.Determine the anti-HBs neutralizing antibody by RIA,the subclass of antibody by ELISA,the function of CTLs by ELISPOT,and compare with those of HB vaccine containing aluminium hydroxide adjuvant.Results BCG-CpG-DNA increased the anti-HBs neutralizing antibody level,promoted the production of antigen specific IgG2a,induced Th1 immune response,and partially reversed Th2 response.The killing activity of antigen specific CD8 T lymphocytes of mice in test group was significantly higher than that in control group.Conclusion BCG-CpG-DNA showed good adjuvanticity to recombinant HBsAg,and might be used as a novel immunoadjuvant. |
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| Keywords: | BCG-CpG-DNA HBsAg |
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