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抗氧化低密度脂蛋白单克隆抗体制备方法的研究
引用本文:徐燕华,罗祖秀.抗氧化低密度脂蛋白单克隆抗体制备方法的研究[J].粉末涂料与涂装,2007,20(9):693-696.
作者姓名:徐燕华  罗祖秀
作者单位:成都华神生物技术有限责任公司技术研发部,成都610225
摘    要:目的研究抗氧化低密度脂蛋白(OxLDL)单克隆抗体的制备方法。方法采用小鼠体内法制备抗OxLDL单克隆抗体时,腹水中抗体经SuperoseTM6 HR10/30凝胶柱进行一步纯化;采用细胞培养法时,分别对杂交瘤细胞生长特性和抗体表达条件进行研究,细胞培养上清中抗体经Streamline SPXL阳离子扩张柱床层析和QXL阴离子交换层析纯化。结果小鼠体内法制备的抗体纯度为95.7%,回收率为65.4%;细胞培养上清中得到的抗体纯度为94.7%,回收率为47.8%。结论已建立了2种抗OxLDL单克隆抗体的制备方法,为OxLDL酶联免疫检测试剂盒的研制奠定了基础。

关 键 词:氧化低密度脂蛋白  单克隆抗体  体外培养  体内培养
文章编号:1004-5503(2007)09-693-04
收稿时间:2006-09-22
修稿时间:2006年9月22日

Development of Methods for Preparation of Monoclonal Antibody against Oxidized Low Density Lipoprotein
XU Yan-hua,LUO Zu-xiu.Development of Methods for Preparation of Monoclonal Antibody against Oxidized Low Density Lipoprotein[J].Chinese Journal of Biologicals,2007,20(9):693-696.
Authors:XU Yan-hua  LUO Zu-xiu
Affiliation:Chengdu Hoist Biotechnology Co. Ltd, Chengdu 610225, China
Abstract:Objective To develop the methods for preparation of monoclonal antibody(McAb) against oxidized low density lipoprotein(OxLDL). Methods Prepare McAb against OxLDL by in vivo culture in mice and purify by SuperoseTM 6 HR10/30 gel column chromatography.Meanwhile,prepare the McAb by in vitro cell culture,observe the growth of hybridoma cells and explore the condition for expression of McAb,then purify the McAb in cell culture supernatant by Streamline SPXL cation expanded bed chromatography and QXL anion exchange chromatography.Results The purity and recovery of McAb prepared by in vivo culture in mice were 95.7% and 65.4%,while those by in vitro cell culture were 94.7% and 47.8% respectively.Conclusion Two methods for preparation of McAb against OxLDL were developed,which laid a foundation of development of ELISA kit for OxLDL.
Keywords:Oxidized low density lipoprotein(OxLDL)  Monoclonal antibody  In vitro culture  In vivo culture
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