Alterations of high density lipoprotein subclasses in obese subjects |
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Authors: | Li Tian Lianqun Jia Mingde Fu Ying Tian Yanhua Xu Haoming Tian Yuye Yang |
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Affiliation: | (1) Laboratory of Endocrinology and Metabolism, West China Hospital, Sichuan University, Chengdu, 610041 Sichuan, People's Republic of China;(2) Department of Biochemistry, Liaoning University of Traditional Chinese Medicine, Shenyang Liaoning, PR China;(3) Department of Biochemistry and Molecular Biology, Nanhua University, Hengyang, Human, PR China;(4) Hoist Group Postdoctoral Work Station, Chengdu, Sichuan, PR China |
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Abstract: | The object of this study was to investigate the characteristics of lipid metabolism in obese subjects, with particular emphasis
on the alteration of HDL subclass contents and distributions. A population of 581 Chinese individuals was divided into four
groups (25 underweight subjects, 288 of desirable weight, 187 overweight, and 45 obese) according to body mass index (BMI).
Apoprotein A-I (apoA-I) contents of plasma HDL subclasses were determined by 2-D gel electrophoresis associated with an immunodetection
method. The concentrations of TG and the apoA-I content of pre-α1-HDL were significantly higher (P<0.01 and P<0.01, respectively), but the levels of HDL cholesterol, and the apoA-I contents of HDL2a and HDL2b were significantly lower (P<0.01, P<0.05, and P<0.01, respectively) in obese subjects than in subjects having a desirable weight. Moreover, with the elevation of BMI, small-sized
pre-α1-HDL increased gradually and significantly, whereas large-sized HDL2b decreased gradually and significantly. Meanwhile, the variations in HDL subclass distribution were more obvious with the
elevation of TG levels in obese as well as overweight subjects. In addition, Pearson correlation analysis revealed that BMI
and TG levels were positively correlated with pre-α1-HDL but negatively correlated with HDL2b. Multiple regression analysis also showed that TG concentrations were associated independently and positively with high pre-α1-HDL and independently and negatively with low HDL2b in obese and overweight subjects. The HDL particle size was smaller in obese and overweight subjects. The shift to smaller
size was more obvious with the elevation of BMI and TG, especially TG levels. These observations, in turn, indicated that
HDL maturation might be abnormal, and reverse cholesterol transport might be impaired.
The first two authors contributed equally to this study. |
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