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| 水苏碱的柱前衍生化HPLC测定 | |
| 引用本文： | 刘玮,邸多隆,曾经泽,陈临熹.水苏碱的柱前衍生化HPLC测定[J].精细化工,2002,19(5):254-256. |
| 作者姓名： | 刘玮 邸多隆 曾经泽 陈临熹 |
| 作者单位： | 1. 河西学院化学系,甘肃张掖734000 2. 张掖地区药检所,甘肃张掖734000 3. 华西医科大学药学院,四川成都610044 |
| 摘 要： | 选择含水苏碱的中药复方制剂前列泰片为对象 ,研究水苏碱的柱前衍生化HPLC测定方法。以对溴基溴化苯乙酮为衍生化试剂 ,18 冠醚 6为相转移催化剂 ,流动相为V(甲醇 )∶V(水 )=75∶2 5 ,检测波长 2 5 4nm ,用外标法定量。线性范围 0 5～ 5 μg(n =5 ) ,相关系数r =0 9992 ,平均回收率 10 0 1% (n =3) ,相对标准偏差 (RSD)为 0 6 4 %。重现性好 ,灵敏度高 ,为中成药中水苏碱的含量测定提供了参考方法。 |
| 关 键 词： | 水苏碱 柱前衍生化 HPLC 含量测定 |
| 文章编号： | 1003-5214(2002)05-0254-03 |
Determination of Stachydrine Concentration by HPLC with Pre-column Derivatization |
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| LIU Ye wei ,DI Duo long ,ZENG Jing ze ,CHEN Lin xi.Determination of Stachydrine Concentration by HPLC with Pre-column Derivatization[J].Fine Chemicals,2002,19(5):254-256. | |
| Authors: | LIU Ye wei DI Duo long ZENG Jing ze CHEN Lin xi |
| Affiliation: | LIU Ye wei 1,DI Duo long 2,ZENG Jing ze 3,CHEN Lin xi 2 |
| Abstract: | To establish the pre column derivatization HPLC method for determinnation of stachydrine concentration, α ,4 dibromoacetophenone was selected as derivatization reagent and 18 crown 6 as phase transfer catalyst.The mobile phase consisted of methanol and water (75∶25).The eluant was monitored at 254 nm.The linear range was 0 5-5 μg( n =5),related coefficient r =0 9992.The average recovery was 100 1%( n =3).Relative standard deviation was 0 64%.This method is sensitive and accurate,and can be used to determine stachydrine content in prepared traditional Chinese medicine. |
| Keywords: | stachydrine pre colum derivatization HPLC |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |