开封产3种白色菊花提取物的抗氧化活性

采用清除二苯代苦味肼基(DPPH)自由基、清除2,2′-连氨-(3-乙基苯并噻唑啉-6-磺酸)二氨盐](ABTS)自由基及铁离子还原/抗氧化能力(FRAP)测定法,对开封产的3种白色菊花(兼六香白、国华万胜及白玉带)不同溶剂提取物的体外抗氧化活性进行了评价,将所测定结果与水溶性维生素E(6-hydroxy-2,5,7,8-tetramethyl-chroman-2-carboxylic acid,Trolox)及阳性对照二丁基羟基甲苯(BHT)进行了比较。结果表明,3种菊花的不同溶剂提取物抗氧化活性不同。同一种菊花的甲醇提取物具有很好清除DPPH自由基和还原铁离子的能力,而石油醚提取物几乎无活性。菊花的3个品种中,兼六香白和国华万胜的抗氧化活性较好,其活性远远超过白玉带的抗氧化活性。9个提取物中,兼六香白的甲醇提取物总的抗氧化活性最好。它对DPPH自由基的清除能力(IC50值为20.49 mg/L)比BHT(IC50值为18.92 mg/L)作用略低;其还原Fe3+的能力(FRAP值为731.73±1.77μmol TE/g)比BHT(1 581.68±97.41μmol TE/g)作用低1/2。3种方法测定结果基本一致,其中以DPPH法和FRAP法相关性最好(R=0.982 0)。

Antioxidant Activity of Extracts from Three Species of White Chrysanthemum morifolium Ramat in Kaifeng

The total antioxidant activity of extracts from three species of Chrysanthemum morifolium Ramat in Kaifeng in vitro,including Jianliuxiangbai,Guohuawansheng and Baiyudai,was assayed by three methods,namely,1,1-diphenyl-2-picryl-2-picrylhydrazyl(DPPH) radical scavenging,2,2′-azino-bis-3-ethylbenzothizaoline-6-sulfonic acid] diammonium salt(ABTS) radical scavenging,and ferric reducing antioxidant power(FRAP) assay.Moreover,the results were compared with 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid(Trolox) and positive control 2,6-Di-tert-butyl-4-methylphenol(BHT).The results showed that different extracts had different activity.In the same species of C.morifolium,the methanol extract generally showed good scavenging activity against DPPH radical and ferric reducing antioxidant power,while the petroleum ether extract hardly had antioxidant activity.In three species of C.morifolium Ramat,Jianliuxiangbai and Guohuawansheng had better antioxidant activity,which was higher than that of Baiyudai.Among the nine extracts,the methanol extract of Jianliuxiangbai exhibited the highest total antioxidant activity.It showed slightly lowered DPPH radical scavenging activity with IC 50 value of 20.49 mg/L than that of BHT with IC 50 value of 18.92 mg/L;and it exhibited ferric reducing antioxidant power with FRAP value of 731.73±1.77 μmol TE/g,that of it was almost half of BHT with FRAP value 1 581.68±97.41 μmol TE/g.The results among the three methods were consistent,and the values obtained by FRAP assay were best correlated with those obtained by DPPH assay(R=0.982 0).