红豆越橘花青素与金银花多酚协同抗氧化活性

探讨了红豆越橘花青素和金银花多酚对DPPH自由基、ABTS自由基、羟基自由基、超氧阴离子自由基的清除及铜离子还原能力和总还原能力的协同抗氧化活性.测定单一组分和复配物〔分别以红豆越橘花青素与金银花多酚按质量比5:1、2:1、1:1、1:2、1:5进行复配,记为复配物(5:1)、(2:1)、(1:1)、(1:2)、(1:5),下同〕抗氧化活性,计算联合作用指数(CI),分析复配物是否具有协同抗氧化作用.结果显示,除复配物(1:5)在清除DPPH自由基上表现为拮抗作用外,其他比例复配物在各项指标中均表现出协同效果.其中,复配物(2:1)对DPPH自由基和铜离子还原能力的CI值分别为0.59±0.07和0.35±0.03,表明二者以质量比2:1复配后对DPPH自由基的清除和铜离子还原能力起到协同作用;复配物(1:1)对ABTS自由基和总还原能力对应的CI值分别为0.58±0.04和0.33±0.04,表明复配后,对ABTS自由基清除及总还原能力具有协同抗氧化作用.红豆越橘花青素和金银花多酚复配具有很强的抗氧化协同功效.

Synergism Antioxidation of Lingonberries Anthocyanin and Lonicera japonica Polyphenols

Explore the synergistic antioxidant capacity of Lingonberries anthocyanin and Lonicera japonica polyphenols. Determination of single component and compound (respectively compounded with Lingonberries anthocyanin and Lonicera japonica polyphenol mass ratio 5:1, 2:1, 1:1, 1:2, 1:5) on DPPH free radical, ABTS free radical, hydroxyl radicals, superoxide anion scavenging ability, copper ion reducing ability and total reduction reaction, calculate the combination index CI, and analyze whether the compound in each proportion has a synergistic antioxidant effect. The results showed that, except the compound (1:5) showed antagonistic effect on scavenging DPPH free radical, the other proportion compound showed synergistic effect in each index. Among them, when the DPPH radical scavenging rate and copper ion reduction capacity of the complex (2:1) reached 50%, CIDPPH=0.59±0.07 and CI copper ion =0.35±0.03, indicating that the complex with mass ratio of 2:1 had a synergistic effect on the scavenging of free radicals and a synergistic antioxidant effect. When the effect of complex (1:1) on ABTS free radical and total reduction was 50%, CIABTS=0.58±0.04, and CI total reduction force =0.33±0.04, indicating that the antioxidant capacity was significantly improved after 1:1 combination. The experiment proved that the combination of Lingonberries anthocyanin and Lonicera japonica polyphenols have synergistic effect on the free radical scavenging and metal ion reduction ability. The results of the study show that the combination of Lingonberry anthocyanin and Lonicera japonica polyphenol has a strong antioxidant synergistic effect, which can balance the cold of honeysuckle, relieve the current situation of large amount of honeysuckle and short supply. It provides a theoretical basis for the utilization and development of natural green and safe antioxidant health products.