核桃仁抗氧化活性研究

采用清除二苯代苦味肼基(DPPH)自由基、清除2,2′-连氨-(3-乙基苯并噻唑啉-6-磺酸)二氨盐](ABTS)自由基及铁离子还原/抗氧化能力(FRAP)测定法,对核桃仁提取物进行抗氧化活性评价,并与阳性对照没食子酸丙酯(PG)、丁基羟基茴香醚(BHA)和二丁基羟基甲苯(BHT)进行比较。在核桃仁提取物中,乙酸乙酯提取物清除DPPH和ABTS自由基的能力(IC50值分别为1.58 mg/L和1.69 mg/L)强于BHA(IC50值分别为3.43 mg/L和1.72 mg/L)和BHT(IC50值分别为18.79 mg/L和6.04 mg/L),弱于PG(IC50值分别为0.86 mg/L和0.66 mg/L);乙酸乙酯提取物还原Fe3+的能力最强〔FRAP值为(13212.99±55.35)μmol TE/g〕,强于PG〔FRAP值为(10617.75±138.38)μmol TE/g〕、BHA〔FRAP值为(7383.10±121.08)μmol TE/g〕和BHT〔FRAP值为(1748.49±3.46)μmol TE/g〕;核桃仁正丁醇提取物清除DPPH和ABTS自由基的能力(IC50值分别为4.94和1.90 mg/L)以及还原Fe3+的能力〔FRAP值为(2299.99±27.68)μmol TE/g〕强于BHT。结果表明,核桃仁乙酸乙酯和正丁醇提取物都具有很好的抗氧化活性,且乙酸乙酯提取物活性强于正丁醇提取物。

Antioxidant Activity of Juglans Regia L.

The antioxidant activities of Juglans regia L.were evaluated by the 1,1-diphenyl-2-picryl-2-picrylhydrazyl(DPPH) radical scavenging,2,2′-azino-bis-3-ethylbenzothizaoline-6-sulfonic acid] diammonium salt(ABTS) radical scavenging,and ferric reducing antioxidant power(FRAP) assay.The results were compared with that of positive controls PG,BHA and BHT.In the two extracts from J.regia L.,the ethyl acetate extract had higher activity of DPPH and ABTS radical scavenging(IC50=1.58 mg/L and IC50=1.69 mg/L respectively) than that of BHA(IC50=3.43 mg/L and IC50=1.72 mg/L) and BHT(IC50=18.79 mg/L and IC50=6.04 mg/L),lower than that of PG(IC50=0.86 mg/L and IC50=0.66 mg/L),the ethyl acetate extract exhibited the highest ferric reducing antioxidant power with FRAP value of(13212.99±55.35) μmol TE/g,higher than that of PG,BHA and BHT(the FRAP values were(10617.75±138.38) μmol TE/g,(7383.10±121.08) μmol TE/g,(1748.49±3.46) μmol TE/g,respectively).The n-butanol extract had a higher DPPH and ABTS radical scavenging activity with IC50 value of 4.94 mg/L and 1.90 mg/L than that of BHT,also showed higher ferric reducing antioxidant power with FRAP value of(2299.99±27.68) μmol TE/g than that of BHT.The results showed that the ethyl acetate and n-butanol extracts had higher antioxidant activities,and the ethyl acetate extract had a higher antioxidant activity than that of n-butanol extract.