有机相酶促合成阿魏酸糖酯

利用酶促方法合成阿魏酸葡糖酯,对酶和有机溶剂进行了比较和筛选,并对影响阿魏酸葡糖酯产率的因素(酶量、反应时间、反应温度、底物比)进行了研究。结果表明,葡萄糖和阿魏酸乙烯酯(摩尔比4∶1)分别加入到体积比2∶1的无水吡啶和叔丁醇中,20 g/L Novozym 435脂肪酶,在220 r/min的空气振荡器中50℃反应72 h,产率能达到80.9%。

Enzymatic Synthesis of Feruloylated Glucoside Ester in Organic Media

A facile enzymatic approach to synthesize feruloylated glucoside derivatives through lipase-catalyzed transesterification of ferulic acid vinyl ester (FAVE) with glucose was developed. The effect of various reaction parameters on the synthesis of feruloylated glucoside was studied systematically. Lipase Novozym 435 was found to have the highest catalytic activity in an anhydrous pyridine/tert-butyl alcohol (2:1, V/V) solvent mixture. FAVE was selectively conjugated onto the primary hydroxyl group of glucose (1:4, n/n), with a yield of 80.9% under optimal conditions.