耳叶紫菀化学成分及生物活性研究

首次对耳叶紫菀(Aster auriculatus Franch)全草的化学成分及生物活性进行研究,采用柱层析(CC)对植物中的提取物进行分离提纯,并利用现代波谱方法(1H-NMR,13C-NMR,MS等)分析鉴定,确定化合物的结构并对其生物活性进行筛选。从该植物的氯仿部分分离得到8个倍半萜类化合物,它们的结构分别鉴定为:Ilicic acid(1),(7R,10S)-selina-4,11(13)-dien-3-on-12-oic acid(2),2-oxo-isocostic acid(3),1β,5α-diangeloyloxy-eudesm-(15)-ene(4),1β,6α-dihydroxyeudesm-4(15)-ene(5),4(15)-eudesmene-1β,7α-diol(6),Furanoligularenone(7),(4αR,5S,8αR)-4α,5,6,7,8,8α-Hexahydro-8α-hydroxy-3,4α,5-trimethylnaphtho2,3-b]furan-2(4H)-one(8)。并采用杯碟法对以上8个化合物进行生物活性筛选,通过观测各供试菌抑菌圈的半径,发现化合物2、化合物4对大肠杆菌(Escherichia coli)有较强的抑制作用,所有化合物均为首次从该植物中分离得到。

Chemical Constituents and Bioactivity of Aster Auriculatus Franch

The chemical constituents and their bioactivity of Aster auriculatus Franch were studied,The whole plant of air-dried Aster auriculatus Franch was extracted with ether-Et2O-MeOH（1：1：1）.The extract was suspend in H2O and extracted with CHCl3 successively.The compounds were isolated and purified by column chromatography from the CHCl3 fraction,and identified based on spectral analyses（1H-NMR,13C-NMR,MS）.Eight compounds isolated from Aster auriculatus Franch were characterized as： ilicic acid（1）,（7R,10S）-selina-4,11（13）-dien-3-on-12-oic acid（2）,2-oxo-isocostic acid（3）,1β,5α-diangeloyloxy-eudesm-（15）-ene（4）,1β,6α-dihydroxyeudesm-4（15）-ene（5）,4（15）-eudesmene-1β,7α-diol（6）,Furanoligularenone（7）,（4αR,5S,8αR）-4α,5,6,7,8,8α-Hexahydro-8α-hydroxy-3,4α,5-trimethylnaphthofuran-2（4H）-one（8）.The bioassays showed weaker sterilization activities for compound 2 and 4.Compounds were all obtained from Aster auriculatus Franch for the first time.