新型糖基阳离子表面活性剂与牛血清白蛋白的相互作用研究

采用荧光光谱法和三维荧光光谱法研究了糖基阳离子表面活性剂N,N-二甲基-N3-(糖酰胺基)]丙基-N-十四烷基溴化铵(C_(14)DGPB)与牛血清白蛋白(BSA)的相互作用。考察了各因素对C_(14)DGPB与BSA相互作用的影响。结果表明,当作用时间为30 min,NaCl的浓度为0.03 mol/L,pH=6.6时,为C_(14)DGPB与BSA相互作用的较佳条件;通过计算可以得到不同温度下C_(14)DGPB对BSA的动态猝灭常数、结合参数和热力学参数;由热力学参数确定它们之间的作用力主要是疏水作用力,对酪氨酸和色氨酸的疏水微环境影响较大。

Study on the interactions between a novel sugar-based cationic surfactant and bovine serum albumin

The interactions between a sugar-based cationic surfactant N,N-dimethyl-N-3-(gluconamide)]propyl-N-alkylammonium bromides (C14DGPB) and bovine serum albumin (BSA) were investigated by fluorescence and three-dimensional fluorescence spectroscopy. The effects of various factors on the interactions between C14DGPB and BSA were investigated. The results show that the optimal conditions for interactions between C14DGPB and BSA were the action time 30 min, 0.03 mol/L NaCl and pH=6.6. The dynamic quenching constants, binding parameters and thermodynamic parameters of C14DGPB with BSA at different temperatures can be obtained by calculation. According to the thermodynamic parameters, it shows that the hydrophobic interaction has played dominant roles in the interactions between C14DGPB and BSA, which has a great influence on the hydrophobic microenvironment of tyrosine and tryptophan.