| HPLC法同步测定三七粉中人参皂苷Rg1、Rb1及三七皂苷R1的含量 |
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| 引用本文： | 李兵,张文成,任少伟,丁肖锋,李如福,李学彬,李广龙.HPLC法同步测定三七粉中人参皂苷Rg1、Rb1及三七皂苷R1的含量[J].安徽化工,2014(3):83-84. |
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| 作者姓名： | 李兵 张文成 任少伟 丁肖锋 李如福 李学彬 李广龙 |
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| 作者单位： | 合肥工业大学农产品生物化工教育部工程研究中心,安徽合肥,230009;安徽信屹康中药工业有限公司,安徽 合肥,230071 |
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| 摘 要： | 目的:采用HPLC法同步测定三七粉中人参皂苷Rg1、Rb1及三七皂苷R1含量。方法:采用SGE protecol C18(5μm,4.6×250mm)色谱柱,流动相为乙腈—水梯度洗脱(0~12min,19∶81;12~60min,19~36∶81~64),检测波长为203nm,流速1.0mL/min,进样量20μL。结果:人参皂苷Rg1、人参皂苷Rb1、三七皂苷R1分别在25.625~430mg/L、26.875~410mg/L、10.625~170mg/L范围呈线性,相关系数r分别为0.9999、0.9998、0.9996;该方法重复性及回收率均符合要求。结论:本法用于同步测定三七粉中人参皂苷Rg1、Rb1及三七皂苷R1的含量,具有简便、准确、高效等特点。
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| 关 键 词： | 人参皂苷Rg1 人参皂苷Rb1 三七皂苷R1 同步检测 |
Synchronous Determination of Ginsenoside Rg1, Ginsenoside Rb1 and Notoginsenoside R1 in Radix Notoginseng Powder by HPLC |
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| LI Bing;ZHANG Wen-cheng;REN Shao-wei;DING Xiao-feng;LI Ru-fu;LI Xue-bin;LI Guang-long.Synchronous Determination of Ginsenoside Rg1, Ginsenoside Rb1 and Notoginsenoside R1 in Radix Notoginseng Powder by HPLC[J].Anhui Chemical Industry,2014(3):83-84. |
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| Authors: | LI Bing;ZHANG Wen-cheng;REN Shao-wei;DING Xiao-feng;LI Ru-fu;LI Xue-bin;LI Guang-long |
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| Affiliation: | LI Bing, ZHANG Wen-cheng, REN Shao-wei, DING Xiao-feng, LI Ru-fu, LI Xue-bin, LI Guang-long ( 1.Engineering Research Center of Bio-process from Ministry of Education, Hefei University of Technology, Hefei 230009, China; 2.Anhui Xinyikang Traditional Chinese Medicine Industry Co., Ltd., Hefei 230071, China) |
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| Abstract: | Objective： This study has been to establish a method for synchronous determination of ginsenoside Rg1, ginsenoside Rb1 and notoginsenoside R1 in radix notoginseng powder. Method： SGE protecol C18（5μm,4.6×250mm）chromatographic column was used, with mixtures of acetonitrile and water as mobile phase in gradient mode （0-12min,19∶81; 12-60min, 19-36∶81-64）, the wavelength was 203nm, the flow rate was 1.0mL/min, the injection volume was 20μL. Results： The linear range of the three saponins is 25.625 - 430mg/L, 26.875 - 410mg/L, 10.625 - 170mg/L respectively, and the correlation coefficient r is 0.9999, 0.9998, 0.9996 respectively. Repeatability and recovery rate are good. Conclusion： The presented method is a simple, accurate, efficient method for synchronous determination ginsenoside Rg1, ginsenoside Rb1 and notoginsenoside R1 in radix notoginseng powder. |
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| Keywords: | ginsenoside Rg1 ginsenoside Rb1 notoginsenoside R1 synchronous determination |
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