| 利用基元模式分析酿酒酵母的葡萄糖厌氧发酵过程以提高乙醇产量 |
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| 引用本文： | 许晓菁,曹利民,陈询.利用基元模式分析酿酒酵母的葡萄糖厌氧发酵过程以提高乙醇产量[J].中国化学工程学报,2008,16(1):135-142. |
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| 作者姓名： | 许晓菁 曹利民 陈询 |
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| 作者单位： | Department of Biochemical Engineering, School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, China |
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| 摘 要： | Elementary flux mode （EFM） analysis was used in the metabolic analysis of central carbon metabolism in Saccharomyces cerevisiae based on constructed cellular network. Calculated from the metabolic model, the ethanol-producing pathway No. 37 furthest converts the substrate into ethanol among the 78 elementary flux modes. The in silico metabolic phenotypes predicted based on this analysis fit well with the fermentation performance of the engineered strains, KAM3 and KAMll, which confirmed that EFM analysis is valid to direct the construction of Saccharomyces cerevisiae engineered strains, to increase the ethanol yield.
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| 关 键 词： | elementary flux mode analysis metabolic phenotype redox balance Saccharomyces cerevisiae ethanol |
| 收稿时间： | 2007-04-19 |
| 修稿时间： | 2007-08-11 |
Elementary Flux Mode Analysis for Optimized Ethanol Yield in Anaerobic Fermentation of Glucose with Saccharomyces cerevisiae |
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| Xiaojing XU, Limin CAO,Xun CHEN.Elementary Flux Mode Analysis for Optimized Ethanol Yield in Anaerobic Fermentation of Glucose with Saccharomyces cerevisiae[J].Chinese Journal of Chemical Engineering,2008,16(1):135-142. |
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| Authors: | Xiaojing XU Limin CAO Xun CHEN |
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| Affiliation: | Department of Biochemical Engineering, School of Chemical Engineering & Technology, Tianjin University, Tianjin 300072, China |
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| Abstract: | Elementary flux mode (EFM) analysis was used in the metabolic analysis of central carbon metabolism in Saccharomyces cerevisiae based on constructed cellular network. Calculated from the metabolic model, the ethanol-producing pathway No. 37 furthest converts the substrate into ethanol among the 78 elementary flux modes.The in silico metabolic phenotypes predicted based on this analysis fit well with the fermentation performance of the engineered strains, KAM3 and KAM11, which confirmed that EFM analysis is valid to direct the construction of Saccharomyces cerevisiae engineered strains, to increase the ethanol yield. |
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| Keywords: | elementary flux mode analysis metabolic phenotype redox balance Saccharomyces cerevisiae ethanol |
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