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| 高酒精耐受性产酒酵母菌的选育 | |
| 引用本文： | 姚小飞,吴晓慧,叶璐.高酒精耐受性产酒酵母菌的选育[J].化学与生物工程,2013,30(7):67-70. |
| 作者姓名： | 姚小飞 吴晓慧 叶璐 |
| 作者单位： | 1. 武汉生物工程学院生物工程系,湖北武汉,430415 2. 华中农业大学,湖北武汉,430205 3. 武汉市汉铁高级中学,湖北武汉,430010 |
| 摘 要： | 从某高粱酿酒厂的酒糟中分离到．1株耐受19％酒精的具有产酒精能力的酵母菌，标记为S17，初步鉴定其属于酒香酵母属（Brettanomyces）。对菌株S17进行紫外诱变以提升其耐酒精的能力。结果表明，菌株S17经紫外线照射120s，筛选得到一株可以耐受20％酒精的正突变株，相比原始菌株的酒精耐受力提高了1％。 |
| 关 键 词： | 酵母菌 酒精耐受 紫外诱变 |
Breeding of A Yeast Producing Alcohol with High Alcohol Tolerance |
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| YAO Xiao-fei , WU Xiao-hui , YE Lu.Breeding of A Yeast Producing Alcohol with High Alcohol Tolerance[J].Chemistry & Bioengineering,2013,30(7):67-70. | |
| Authors: | YAO Xiao-fei WU Xiao-hui YE Lu |
| Affiliation: | 1.Department of Bioengineering,Wuhan Bioengineering Institute,Wuhan 430415,China; 2.Huazhong Agricultural University,Wuhan 430205,China; 3.Wuhan Hantie Middle School,Wuhan 430010,China) |
| Abstract: | A yeast producing alcohol with high alcohol tolerance was screened from lees and named as S17. This strain was tentatively identified as Brettanomyces. The mutation of strain S17 by ultraviolet was carried out to improve its alcohol tolerance. The result showed that,with the ultraviolet mutagenesis for 120 s,a posi-tive mutant was obtained,which could tolerate 20% （volume fraction） alcohol, enhancing 1% compared to that of the original strain. |
| Keywords: | yeast alcohol tolerance ultraviolet mutagenesis |
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