| 双水相体系萃取木瓜蛋白酶的研究 |
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| 引用本文： | 乐薇,陆志强.双水相体系萃取木瓜蛋白酶的研究[J].化学与生物工程,2011,28(2):40-42. |
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| 作者姓名： | 乐薇 陆志强 |
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| 作者单位： | 中南民族大学工商学院,环境与生命科学系,湖北,武汉,430065 |
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| 摘 要： | 采用聚乙二醇(PEG)/(NH4)2SO4双水相体系对木瓜蛋白酶进行萃取分离,研究了PEG相对分子量、PEG质量分数、(NH4)2SO4质量分数和pH值对木瓜蛋白酶分配系数及酶活力回收率的影响.结果表明,最佳萃取条件为:PEG4000质量分数6%、(NH4)2SO4质量分数18%、pH值6.0,在此条件下,木瓜蛋白酶的...
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| 关 键 词： | 双水相体系 萃取 木瓜蛋白酶 |
Study on Extraction of Papain by Aqueous Two-Phase System |
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| YUE Wei,LU Zhi-qiang.Study on Extraction of Papain by Aqueous Two-Phase System[J].Chemistry & Bioengineering,2011,28(2):40-42. |
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| Authors: | YUE Wei LU Zhi-qiang |
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| Affiliation: | YUE Wei,LU Zhi-qiang(Department of Environment and Life Science,Engineering and Commerce College ofSouth-Central University for Nationalities,Wuhan 430065,China) |
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| Abstract: | The extraction of papain by aqueous two-phase system formed by polyethylene glycol(PEG) and(NH4)2SO4 was studied.The effects of relative molecular weight and mass fraction of PEG,mass fraction of(NH4)2SO4 and pH value on the distribution coefficient,activity recovery rate were examined.The results showed that the optimum extraction conditions were PEG4000 mass fraction 6%,(NH4)2SO4 mass fraction 18%,and pH value 6.0.Under above conditions,the activity recovery rate and distribution coefficient reached 80.25... |
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| Keywords: | aqueous two-phase system extraction papain |
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