| 大鼠血栓调节蛋白基因引物的设计与研究 |
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| 引用本文： | 陈秋晨,王琳,陈再兴,朱旭,张岐山,孟繁浩. 大鼠血栓调节蛋白基因引物的设计与研究[J]. 化学与生物工程, 2009, 26(1) |
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| 作者姓名： | 陈秋晨 王琳 陈再兴 朱旭 张岐山 孟繁浩 |
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| 作者单位： | 中国医科大学药学院,辽宁,沈阳,110001;中国医科大学药学院,辽宁,沈阳,110001;中国医科大学药学院,辽宁,沈阳,110001;中国医科大学药学院,辽宁,沈阳,110001;中国医科大学药学院,辽宁,沈阳,110001;中国医科大学药学院,辽宁,沈阳,110001 |
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| 基金项目： | 辽宁省百千万人才工程培养经费资助项目 |
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| 摘 要： | 设计了大鼠血栓调节蛋白基因PCR扩增引物,并对引物与模板的加入量及退火温度等进行了研究,优化了PCR反应.结果表明,PCR反应所得产物电泳条带清晰,位置正确,无非特异性扩增带;用该方法设计得到的基因引物能够高效、特异性地进行PCR反应.
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| 关 键 词： | 血栓调节蛋白 基因引物 设计与研究 PCR反应 |
Design and Study of the Primers of Thrombomodulin in Rat |
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| CHEN Qiu-chen,WANG Lin,CHEN Zai-xing,ZHU Xu,ZHANG Qi-shan,MENG Fan-hao. Design and Study of the Primers of Thrombomodulin in Rat[J]. Chemistry & Bioengineering, 2009, 26(1) |
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| Authors: | CHEN Qiu-chen WANG Lin CHEN Zai-xing ZHU Xu ZHANG Qi-shan MENG Fan-hao |
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| Affiliation: | School of Pharmacy;China Medical University;Shenyang 110001;China |
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| Abstract: | A pair of primers of thrombomodulin in rat for PCR reaction were designed and studied.The PCR reactions were optimized by adjusting the dose of primers or template and the renaturation temperature.The electrophoresis result of PCR production was satisfactory with clear strap,correct place and non-specificity amplification.The best condition for PCR response was studied,and the PCR reaction had high ability and specificity. |
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| Keywords: | thrombomodulin primer design and study PCR reaction |
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