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复合酶制备葛根粉的工艺研究
引用本文:涂招秀,罗斌,李雄辉,胡居吾.复合酶制备葛根粉的工艺研究[J].江西化工,2013(3):76-79.
作者姓名:涂招秀  罗斌  李雄辉  胡居吾
作者单位:江西省科学院;江西省产品质量监督检测院
摘    要:以葛根浆为酶促反应底物,考察纤维素酶的用量、酶解时间、反应温度对葛粉得率的影响,同时考察了果胶酶以及纤维素酶和果胶酶联合使用对葛粉得率的影响.结果表明,复合酶酶解的最佳工艺参数为每克葛根浆(10%葛粉)中,纤维素酶60-80U,果胶酶20-30U,酶解时间1.5h,反应温度45℃,在此条件下,葛粉量达到19.12%,增加率提高了48.65%,效果远优于分别单独使用各酶,同时又节省了各酶的用量.

关 键 词:葛根  复合酶酶解

Preparation of kuzdu vine with mixed-enzymes
TU Zhao-xiu;LUO Bin;LI Xiong-hui;HU Ju-wu.Preparation of kuzdu vine with mixed-enzymes[J].Jiangxi Chemical Industry,2013(3):76-79.
Authors:TU Zhao-xiu;LUO Bin;LI Xiong-hui;HU Ju-wu
Affiliation:TU Zhao-xiu;LUO Bin;LI Xiong-hui;HU Ju-wu;Jiangxi Academy of Sciences;Jiangxi Institute of Supervision and Inspection on Product Quality;
Abstract:The extraction of Kuzdu vine fromkudzu root was studied in this paper and kuzdu vine was extracted by mixed - enzymes and the mathematical model of correlation among enzyme dosage , extraction temperature, extraction time were established. The results indicated that the optimum extrac(on conditions are as follows:in kudzu root pulp powder ( 10% ) per gram, cellulase dosage 60 - 80U, pectinase dosage 20 - 30U, extraction temperature 45 ℃, extraction time 1.5 h. Under the above conditions,the amount of Kuzdu vine 19. 12% ,the Extraction yield increased 48.65%.
Keywords:Kudzu root mixed - enzymes
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