| 淫羊藿总黄酮提取物树脂纯化工艺条件的优化 |
| |
| 引用本文： | 周国保,张文清.淫羊藿总黄酮提取物树脂纯化工艺条件的优化[J].江西化工,2014(1):84-89. |
| |
| 作者姓名： | 周国保 张文清 |
| |
| 作者单位： | [1]江西省化学工业学校,江西南昌330012 [2]华东理工大学化学与分子工程学院,上海200237 |
| |
| 摘 要： | 以D1400大孔树脂、HZ-841大孔树脂、PA03060聚酰胺树脂,通过动态吸附与洗脱,对淫羊藿总黄酮提取物进行纯化,HZ-841大孔树脂的纯化效果最优。通过正交试验得到最佳纯化工艺条件为:2g HZ-841大孔树脂,上样总黄酮含量2mg/mL的提取液30mL,分别以80mL水、40mL 20%乙醇、40mL85%乙醇和20mL 95%乙醇进行梯度洗脱,流速1.5mL/min。最优条件下,利用HZ-841树脂纯化淫羊藿总黄酮提取液,得到干浸膏收率3.25%,其中总黄酮含量71.8%,淫羊藿苷含量9.44%。
|
| 关 键 词： | 淫羊藿 总黄酮 提取物 大孔树脂 纯化 |
Optimization of Resin Purification technology of Flavonoids from Epimedium |
| |
| Affiliation: | ZHOU Guo - bao ZHANG Wen - qing (1. Jiangxi Province Chemical Industry School,Jiangxi Nanchang 330012; 2. College of Chemistry and Molecular Engineering, East China University of Science and Technology,Shanghai 200237 ) |
| |
| Abstract: | Contrast D1400, HZ -841, PA03060 polyamide resin for harrenwort flavonoids dynamic adsorption and dution effect, HZ - 841 is optimal. Purification conditions were obtained by orthogonal experiment as fellows ：2 g HZ - 841 macro-porous resin was used to purify 30 mL solution of flavonoids content of 2mg/mL , 80mL water, dOtaL 20 % ethanol, dOtaL 85 % ethanol, 20mL 95 % ethanol gradient elution , flow rate 1.5mL/min. Under the optimized conditions, flocculation solution was purified by HZ - 841 resin,the content of total Flavonoids was 71.8% in the extract, and Icariin content was 9.44%, dry extract of Epimedium Pieces yield was 3.25%. |
| |
| Keywords: | Barrenwort flavonoids extract macroporous resin puritlcation |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
