Betacyanins extraction from Hylocereus polyrhizus using alcohol/salt-based liquid biphasic partitioning system and antioxidant activity evaluation |
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| Authors: | Hui Yi Leong Chien Wei Ooi Chung Lim Law Advina Lizah Julkifle |
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| Affiliation: | 1. Bioseparation Research Group, Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Semenyih, Selangor Darul Ehsan, Malaysia;2. Chemical Engineering Discipline, School of Engineering, Monash University Malaysia, Bandar Sunway, Selangor Darul Ehsan, Malaysia;3. Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Semenyih, Selangor Darul Ehsan, Malaysia;4. Crops For the Future, Semenyih, Selangor Darul Ehsan, Malaysia |
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| Abstract: | Betacyanins have gained increasing interest as natural food dye because of their health-promoting effects. This study investigated the betacyanins extraction from peel and flesh of red–purple pitaya using ethanol/K2HPO4-based liquid biphasic partitioning system (LBPS) by optimised concentrations of ethanol, K2HPO4, NaCl and crude. Overall, the highest values of betacyanins concentration in alcohol-rich top phase (Ct), phase volume ratio (Vr) and partition coefficient (K) of betacyanins that represented an optimum betacyanins extraction from the peel (98.080%, 1.667 and 51.097, respectively) and flesh (96.256%, 2.167 and 25.764, respectively) were achieved. Lastly, conventional solvent extractions, colour characterisation and antioxidant activity evaluation were conducted. |
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| Keywords: | Antioxidant activity betacyanins liquid biphasic partitioning system red–purple pitaya |
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