Evaluation of Nitrogen Fixing Enzyme Activities in Response to Pyrene Bioremediation Efficacy by Defined Artificial Microalgal-Bacterial Consortium of Gujarat,India

This study was carried out to investigate the response and relationship between nitrogen fixing enzymes during the remediation of different concentrations of high molecular weight four rings Polynuclear Aromatic Hydrocarbon (PAH) Pyrene by microalgae Synechocystis sp. (cyanobacteria) with artificial developed indigenous bacterial consortium. One axenic microalgal culture of Synechocystis sp. and two pyrene degrading indigenous bacteria with efficient removal capabilities viz. Pseudomonas indoxyladons and Bacillus benzoevorans isolated from crude oil polluted site and common industrial effluent canal were used to construct the consortium. The effect of pyrene on algal growth in terms of chlorophyll-a was measured and it was found that in the presence of bacteria, the growth and bioremediation capacity of Synechocystis sp. raised tremendously, whereas Synechocystis sp. monoculture exhibited concentration dependent decrease. Moreover, the nitrogen fixing enzymes; nitrate reductase (NR), glutamine synthetase (GS), and succinate dehydrogenase (SDH) showed chronological decrease by 93%, 90%, and 98%, respectively. Increased Bioremediation of pyrene by consortium JPNKA7B2 (Mix culture of Synechocystis sp., Pseudomonas indoxyladons, and Bacillus benzoevorans) was eliminated at 94.1% in 50 mg/L, which indirectly retarded the nitrogen fixing enzymes – NR, GS, and SDH. However, Synechocystis sp. monoculture could remediate up to 36% at 1.5 mg/L after 16 days of incubation.