高产精氨酸的钝齿棒杆菌在高低供氧条件下的差异蛋白质组学初步研究

溶氧水平直接影响钝齿棒杆菌合成精氨酸产量的高低,运用双向电泳结合MALDI-TOF-MS及MS/MS质谱鉴定技术的方法对高低供氧条件下高产精氨酸的钝齿棒杆菌的胞内可溶性蛋白进行分离鉴定.结果表明,钝齿棒杆菌菌体蛋白的等电点主要分布在4-7的范围内,采用pH4-7的IPG胶条进行分离,得到了645±12个蛋白点.通过软件...

A preliminary study on differential proteomics of Corynebacterium crenatum in two oxygen supply models

Dissolved oxygen level directly affects the synthesis of arginine by Corynebacterium crenatum(C.crenatum).Proteomic approaches including two dimensional electrophoresis(2 DE)and matrix assisted laser desorption/ionization time of flight mass spectrometry(MALDI TOF MS)were used to analyze differential expression of proteins in C.crenatum in different oxygen supply conditions.The results showed that most of the isoelectric points of the proteins on the 2 DE gels distributed from 4 to 7.Thirty two different protein points were detected in the pH 4—7 gels and characterized by MALDI TOF MS as well as peptide mass fingerprintings.Using matrixscience database, 29 protein points were identified, and they represented 27 proteins.Database research showed that the proteins performed different functions in carbohydrate metabolism, amino acid metabolism and energy metabolism.Most of the differentially expressed proteins concentrated in the pentose phosphate pathway, TCA cycle and the urea cycle.The results showed that in the high oxygen supply model, energy metabolism was significantly increased, and the oxidative stress was balanced because of the increase of pentose phosphate pathway.Meanwhile, the activation of TCA cycle and urea cycle were beneficial to the synthesis of arginine.